细菌P-FS08的鉴定及其对几种植物病原真菌的拮抗作用  

Identification of bacterium P-FS08 and its inhibition to some pathogenic fungi

作　　者：石志琦[1] 胡梁斌[2] 于淑池[3] 徐朗莱[2] 范永坚[1] 

机构地区：[1]江苏省农业科学院食品质量安全与检测研究中心,江苏南京210014 [2]南京农业大学生命科学学院,江苏南京210095 [3]河北大学生命科学学院,河北保定071002

出　　处：《南京农业大学学报》2005年第3期48-52,共5页Journal of Nanjing Agricultural University

基　　金：江苏省"十五"攻关项目(BE2001328);国家863计划项目(2002AA244041)

摘　　要：从油菜茎秆内油菜菌核病菌(Sclerotiniasclerotiorum)菌核上分离得到拮抗菌P FS08,根据其形态特征和生理生化特性以及该细菌的16SrDNA部分序列的进化树分析,鉴定其为多粘芽孢杆菌(Paenibacilluspolymyxa)。经平皿测试表明,多粘芽孢杆菌P FS08菌株及其无菌滤液对烟草赤星病菌(Alternariaalternata)、小麦赤霉病菌(Fusariumgraminea rum)等多种植物病原真菌均有抑制作用。P FS08培养液通过70%硫酸铵沉淀得到具有拮抗活性的蛋白粗提液,其具有良好的热稳定性;对蛋白酶K和胃蛋白酶均不敏感;能够使小麦赤霉病菌菌丝断裂,原生质浓缩。Bacterium P-FS08 was isolated from the nucleus of Sclerotinia sclerotiorum infecting the rape stem and identified as PaenibaciUus polymyxa according to its morphological, physiological, biochemical characters and the analysis of the partial sequence of the 16S rDNA. Both the bacterial culture and aseptic filtrate showed activities to inhibit some pathogenic fungi including Alternaria alternata, and Fusarium graminearum on activity plates assay. Antagonistic crude protein extract was obtained by 70% ammonium sulphate precipitation, and it was thermostable, unsensible to proteinase K and pepsin, and capable to induce the hypha lysis and cytoplasm condensation of F. graminearum.

关 键 词：细菌鉴定 多粘芽孢杆菌 16S RDNA 病原真菌 拮抗作用 

分 类 号：S432.22[农业科学—植物病理学] S332.2[农业科学—农业昆虫与害虫防治]