大鼠肾脏缺血再灌注损伤与重组成骨蛋白-1的关系  

Influence of rOP-1 on ischemia-reperfusion injury in rat kidneys

作　　者：孙立江[1] 李延江[1] 吕振华[2] 相爱兰[1] 于瑞兰[1] 李玉军[3] 

机构地区：[1]青岛大学医学院附属医院泌尿外科,266003 [2]青岛大学医学院附属医院中心实验室,266003 [3]青岛大学医学院附属医院药理科,266003

出　　处：《中华实验外科杂志》2005年第9期1046-1047,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨鼠重组成骨蛋白1(rOP1)在大鼠肾脏缺血再灌注损伤过程中对氧自由基、细胞凋亡的影响及其对肾脏的保护作用。方法观察静脉应用rOP1250μg/kg体重,大鼠肾脏缺血60min,再灌注24h后,血浆超氧化物歧化酶(SOD)、脂质过氧化物丙二醇(MDA)、内皮素1(ET1)的变化,及其对肾脏细胞凋亡的影响。结果rOP1组和缺血再灌注组血浆SOD分别是(108±20)和(86±11)KNU/L,MDA分别是(8.90±0.49)和(11.70±0.81)μmol/L,ET1分别是(130±39)和(179±45)ng/L,rOP1组SOD水平显著性高于缺血再灌注组(P<0.05),而MDA和ET1水平显著性低于缺血再灌注组(P<0.05)。两组肾脏细胞凋亡指数分别是(5.72±3.08)%和(8.85±3.52)%,rOP1组显著性低于缺血再灌注组(P<0.01)。上述两组肾脏的的Miller’s评分分别是(2.0±0.3)和(3.4±0.5),rOP1组显著低于缺血再灌注组(P<0.01)。结论rOP1减轻氧自由基对肾脏缺血再灌注损伤,降低肾脏细胞凋亡指数,对肾脏缺血再灌注损伤具有保护作用。Objective To investigate the influence of rOP-1 on oxygen-delived free radicals and renal cell apoptosis in rat kidneys with .ischemia-reperfusion injury and it＇s protective effect on injured kidneys. Methods The changes of plasma superoxide dismutase （SOD）, malondialdehyde （MDA） and endothelin-1 （ET-1） as well as the influence of 250μg·kg-1 of rOP-1 applied intravenously on renal cell apoptosis were observed 24 hours after reperfusion in rat kidneys of ischemia for 60 minutes. Results The levels of plasma SOD, MDA and ET-1 in rOP-1 group were （ 108±20） KNU/L, （8.90±0.49）μmol/L and （130±39 ） ng/L, respectively and those in ischemia-reperfusion group were （86±11 ） KNU/L, （ 11.70±0.81 ） /μmol/L and （ 179±45 ） ng/L, respectively. The level of SOD in rOP-1 group was significantly higher than that in ischemia-repeffusion group （P〈0.05）, but the levels of plasma MDA and ET-1 were significantly lower than those in ischemia-reperfiasion group （P〈0.05）. The indexes of renal cell apoptosis in the two groups were （5.72±3.08） % and （8.85±3.52） %, respectively and index of renal cell apoptosis in rOP-1 group was significantly decreased than that of ischemia-reperfusion group （ P〈0.01）. The Miller＇s scorings of both two group mentioned above were （2.0±0.3） and （3.4±0.5） and the scoring of rOP-1 group was significantly lower than that of is chemia-reperfusion group （P〈0.01）. Conclusion rOP-1 diminishs renal ischemia-reperfusion injury induced by oxygen-delived free radicals, decreases index of renal cell apoptosis and possesses protective effect on kidney with ischemia- reperfusion injury.

关 键 词：肾脏 缺血再灌注损伤 重组成骨蛋白-1 肾脏缺血再灌注损伤 成骨蛋白-1 超氧化物歧化酶(SOD) 内皮素-1(ET-1) 大鼠 重组 细胞凋亡指数 

分 类 号：R692[医药卫生—泌尿科学] R977.6[医药卫生—外科学]