新西兰兔尿道海绵体平滑肌细胞的原代培养及鉴定  

Primary culture and identification of New Zealand rabbit corpus spongiosum smooth muscle cells

作　　者：张金明[1] 黄红军[1] 何涛[1] 

机构地区：[1]中山大学附属第二医院整形外科,广州510120

出　　处：《中华实验外科杂志》2005年第9期1051-1052,共2页Chinese Journal of Experimental Surgery

基　　金：广东省社会发展基金资助项目(2004B3401003)

摘　　要：目的为构建新西兰兔组织工程尿道提供种子细胞。方法取新西兰兔阴茎头,用含10%胎牛血清的高糖DMEM作体外原代培养,并行免疫组织化学法鉴定。结果经10～14d培养后,培养瓶底铺满梭状细胞,免疫组织化学法鉴定确认为兔尿道海绵体平滑肌细胞。结论兔阴茎头平滑肌细胞可在体外条件下生长传代、扩增,可作为构建组织工程尿道的种子细胞。Objective To provide an ideal seed cells for tissue-engineered urethra. Methods Smooth muscle cells of corpus spongiosum was cultured from New Zealand rabbits＇ glans with DMEM （10 % fetal bovine serum） in vitro and the cultured cells were identified using immunohistochemistry. Rsults After 10-14 days, spindle cells spreaded over the bottom of culture flasks and they were confirmed as smooth muscle cells of corpus spongiosum by immunohistochemistry. Conclusion Smooth muscle cells of corpus spongiosum could be cultured in vitro and proliferated. These cultured cells could serve as seed cells for tissue-engineered urethras.

关 键 词：平滑肌 尿道海绵体 细胞培养 兔 体外原代培养 平滑肌细胞 新西兰兔 鉴定 免疫组织化学法 构建组织工程 

分 类 号：R318.0[医药卫生—生物医学工程]