人体口腔粘膜上皮细胞体外培养和鉴定  

Serial cultivation of normal human buccal epithelium

作　　者：陈文 李森恺 史宏道 李养群 蒋华[2] 贾文英[2] 祝君梅[2] 

机构地区：[1]医学科学院整形外科医院,北京100041 [2]天津市医药科学研究所

出　　处：《现代口腔医学杂志》2005年第5期489-491,共3页Journal of Modern Stomatology

基　　金：国家自然科学基金资助项目(编号:30070779)

摘　　要：目的建立稳定的人口腔颊粘膜上皮细胞体外培养方法.方法取正常人口腔颊粘膜,中性蛋白酶和胰蛋白酶消化后,获取的粘膜上皮细胞接种于10%含胎牛血清培养基中连续培养,探索最适培养条件,进行细胞定性及形态学观察.结果体外培养细胞中无成纤维细胞混杂,长满后呈上皮细胞特有的铺路石样外观,体外可连续传9～10代,生长期50～60天.细胞角蛋白免疫组化染色阳性,透射电镜下可见上皮细胞间特有的桥粒结构.结论采用含血清培养基体外连续培养口腔粘膜上皮细胞获得成功,4代以内培养上皮细胞形态结构与原代无差别,可用于组织工程化口腔粘膜构建.Objective To establish a method of culturing normal human buecal epithelium. Methods Specimens from healthy human oral cavity were dissociated into single cell suspension by dispase and trypsin. The cells were grown in a medium with 10% fetal bovine serum to observe morphological characteristics and the best qualification of cells growing. Results The cells could survive for 50 - 60 days in the culture after passing 9 - 10 generations. The observation of electron microscope revealed that desmosomes between the cells showed positive staining for cytokeratin antibody. Conclusion The culture technique with fetal bovine serum has been established for growing normal human buecal epthelium. The culturing cells anterior 4 passage could be used in oral epithelium tissue engineer.

关 键 词：细胞培养 上皮细胞 口腔粘膜 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]