氢离子选择性液膜超微电极的研制  

Hydrogen lon-selective Liquor Membrane Supermicroelectrode

作　　者：吴芯芯[1] 赵平三 漆德瑶[1] 

机构地区：[1]上海大学化学化工系,上海200072

出　　处：《分析化学》1996年第2期230-233,共4页Chinese Journal of Analytical Chemistry

基　　金：中国科学院长春应用化学研究所电分析化学开放研究实验室资助

摘　　要：本文报道新的以三十二烷基胺（TDDA）为载体和以对氯苯硼四-十二铵（ETH2191）为载体的pH微电极膜。与原有的TDDA膜相比较，新的TDDA膜和ETH2191膜制备更加容易，膜内阻从原有的2×1011Ω0（1μm）下降到3．5×1010Ω（1μm），其中ETH2191膜的动力学响应pH范围为12～4．5，与原有的TDDA膜（pH范围为12～5．5）比较，拓宽了五个pH，而且ETH2191膜无TDDA膜所具有的超能斯特响应。以新TDDA膜和ETH2191膜制成的超微电极的膜内阻仅为1×1011Ω（0．5μm），响应快，能够满足细胞内pH测定的需要。A new tri-n-dodecylamine (TDDA)-based and a ETH2191-based hydrogen ion-selective microelectrodes are described. Comparcd with the old TDDA-based pH microelectrode, these two new microelectrodes can be more easily prepared, and the resistance of both membranes is much lower. The ETH219l-based microelectrode exhibits a linear dynamic response pH range from 12 to 4. 5 and extends 1 pH unit toward the more acidic range, compared with the linear pH range of 12~5. 5 of the former TDDA-based one. The resistance of either the ETH2191-based or the new TDDA-based microelectrode with 0. 5μm diameter is about 1011Ω and thus the new microelectrodes are suitable for physiological measurements, especially for intracellular measurements.

关 键 词：微电极 TDDA 氢离子 PH测定 

分 类 号：O657.92[理学—分析化学]