人隐静脉血管内皮细胞的分离、培养及鉴定  

In Vitro Culture of Human Saphenous Vein Endothelial Cells

作　　者：李斌[1] 刘宁[2] 

机构地区：[1]云南大学药学院生物制药创新人才培养基地,昆明650091 [2]云南省第一人民医院产科,昆明650032

出　　处：《细胞生物学杂志》2005年第4期474-476,共3页Chinese Journal of Cell Biology

基　　金：云南大学理(工)科级科研项目资助(No.K1059069)~~

摘　　要：利用冠脉搭桥术后遗弃的隐静脉段获取内皮细胞,采用消化酶消化收集内皮细胞,扩增、冻存、复苏,在体外建立内皮细胞系。此方法简便易行,能在体外获得大量生物学特性保持良好的内皮细胞,为临床血管内皮化研究提供新的细胞来源。The human saphenous vein endothelial cells were successfully dissociated and cultured by using the human saphenous vein segments leftover from cardiac bypass surgery. Endothelial cells were received, proliferated, frozen, and resuscitated by using digestive enzyme, The content of living cells produced using this method is very high and the passage cells were similar to the primary culture in shape and on the growth rate. It was also shown that endothelial cells line in vitro can be established simply and timely using this method.

关 键 词：隐静脉 血管内皮细胞 细胞培养 静脉 分离 鉴定 生物学特性 细胞来源 消化酶 细胞系 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]