上海市吴淞地区大气PM_(2.5)两种提取成分的细胞毒性研究  

Cytotoxicity of Extracts of PM_(2.5) Collected at Wusong District in Shanghai

作　　者：王伟[1] 程硕[1] 谈明光[1] 陆文忠[1] 童永彭[1] 张桂林[1] 李燕[1] 

机构地区：[1]中科院上海应用物理研究所,上海201800

出　　处：《环境与职业医学》2005年第4期299-302,共4页Journal of Environmental and Occupational Medicine

基　　金：中科院知识创新工程项目(编号:KJCXZ-SN-NO1)国家基金委十五重大项目(编号:1049080)上海市自然科学基金项目(编号:03ZR14111)

摘　　要：[目的]研究上海市吴淞地区大气细颗粒物(PM2.5)两种提取成分(有机和无机提取成分)的细胞毒性。[方法]采用滤膜法采集上海市吴淞地区大气中PM2.5,以中国仓鼠肺成纤维细胞(chinese hamsterfibroblast cell line,CHL) 为靶细胞研究PM2.5有机及无机提取成分对细胞活力、代谢的影响及氧化损伤毒性,具体包括细胞形态、活力和细胞培养液中乳酸脱氢酶(LDH)、乳酸(LD)、丙二醛(MDA)及超氧化物歧化酶(SOD)水平的测定。[结果]上海市吴淞地区大气PM2.5的有机和无机两种提取成分分别以10、50、100、200 μg/ml浓度染毒CHL细胞10 h,结果显示随着染毒剂量的增加细胞形态损伤加剧,细胞存活率、SOD含量呈下降趋势,LD和MDA则呈上升趋势,LDH则呈现先升后降趋势。与阴性对照组相比,200μg/ml染毒组的LD含量的升高同100、200μg/ml组的LDH和MDA含量的升高及SOD水平的下降均具有统计学显著性(P<0.05)。除200μg/ml染毒组,有机提取成分要高于无机提取成分所染毒细胞的生长抑制率外,其他各染毒浓度组的氧化损伤及代谢指标水平在两种染毒成分之间尚不能认为有差别。[结论]上海市吴淞地区PM2.5的有机及无机提取成分均具有一定的细胞毒性。[Objective] This study was undertaken to test the cytotoxicity of extracts, organic and inorganic, of fine particulate matter smaller than 2.5 μm in aerodynamic diameter（ PM2.5 ） gathered at Wusong District, Shanghai. [ Method ] PM2.5 was collected onto teflon filter by middle flux air sampler. CHL cells were treated with both organic and inorganic extracts of PM2.5, at the concentrations from 10 to 200 μg/ml for 10 h, then the oxidation and metabolism of cells including cellular morphology, activity and the concentrations of LDH, LD, MDA, and SOD in cell media were tested. [ Results ] In all experiments, CHL cells were sensitive to exposure to both parts of PM2.5. Based on the results from the laboratory test PM2.5 can metamorphose, oxidize and impair cells and cause cellular metabolic dysfunction. [ Conclusion ] Both organic and inorganic extracts of PM2.5 have some cytotoxicity.

关 键 词：大气颗粒物 PM2.5 细胞毒性 

分 类 号：X513[环境科学与工程—环境工程]