1,2-二氯乙烷对大鼠体外血脑屏障细胞损伤的形态学观察  

Effect of 1, 2-Dichloroethane on Blood Brain-barrier Cells in vitro

作　　者：牛侨[1] 张勤丽[1] 王林平[1] 牛丕业[1] 

机构地区：[1]山西医科大学,山西太原030001

出　　处：《环境与职业医学》2005年第4期303-308,共6页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:30271116)

摘　　要：[目的]进一步探讨1,2-二氯乙烷对血脑屏障细胞的损伤作用。[方法]在体外建立了血脑屏障细胞模型, 并从形态学上观察1,2-二氯乙烷对血脑屏障细胞的损伤作用。体外培养脑微血管内皮细胞和神经胶质细胞,将生长良好的脑微血管内皮细胞和神经胶质细胞分为4组,即对照组、5、10、20μl/ml组,用1,2.二氯乙烷对脑微血管内皮细胞和神经胶质细胞染毒,染毒0、10、30、60 min后于光镜和电镜下密切观察细胞的形态学变化。[结果]染毒后的脑微血管内皮细胞呈进行性破坏,随着染毒剂量的加大细胞体积逐渐增大,胞膜模糊,细胞与细胞间、细胞与突触间连接减少, 甚至消失;随着染毒时间的延长,在10、30、60 min后可以观察到有进行性的细胞膜溶解破坏,胞核模糊,界限不清,突触变短。随着染毒浓度的增加或时间的延长,神经胶质细胞树突数量减少,轴突减少变短,细胞与突触间、胞体与胞体间连接减少甚至消失,超微结构显示胞浆中线粒体膜的破坏、缺失,嵴断裂、肿胀及空泡变,粗面内质网轻度扩张、脱颗粒,细胞核内染色质在核周凝集,胶质细胞间及突触间的连接松解。[结论]1,2-二氯乙烷可造成血脑屏障细胞的损伤,引起血管源性脑水肿。[Objective] To investigate the effect of 1, 2-dichloroethane on blood brain-barrier cells in vitro, brain microvascular endothelial cells and glial cells were cultured as blood brain barrier cells and observed their morphologic structure features. [ Methods ] Brain microvascular endothelial cells and neuroglial cells of newborn rats were cultured in vitro, and treated with 1, 2- dichloroethane at a dose of 0 μl/ml, 5 μl/ml, 10 μl/ml or 20 μl/ml . The cell morphologic structures were observed under light and electron microscope. [ Results ] ① Normal morphological structure of brain microvascular endothelial cells and neuroglial cells were injured by 1,2-diehloroethane , which damaged blood-brain barrier. ② The injury by 1, 2-dichloroethane to neuroglial cells appeared earlier and more seriously than that to brain microvascular endothelial cells. [ Conclusion ] 1,2-dichloroethane can damage blood-brain barrier cells, and induce cerebral edema.

关 键 词：1 2-二氯乙烷 血脑屏障 脑微血管内皮细胞 神经胶质细胞 

分 类 号：R114[医药卫生—卫生毒理学]