煤工尘肺患者血清CuZn-SOD和铜蓝蛋白活性研究  

Studies on Relations between Serum CuZn-SOD and Ceruloplasmin Activities in Patients with Coal Workers' Pneumoconiosis

作　　者：王明臣[1] 胡婵丽[1] 董子明[2] 杨丽萍[3] 张善锋[1] 陈勤 

机构地区：[1]郑州大学基础医学院生化教研室,河南郑州450052 [2]郑州大学基础医学院病生教研室 [3]河南中医学院生化教研室 [4]平顶山煤业集团职业病防治院

出　　处：《环境与职业医学》2005年第4期357-358,共2页Journal of Environmental and Occupational Medicine

基　　金：河南省自然科学基础研究项目(编号:0111024300)河南省高校青年骨干教师资助项目

摘　　要：[目的]探讨煤工尘肺发生发展过程中血清含Cu抗氧化酶CuZn-SOD和铜蓝蛋白(CP)的活性及Cu含量的变化。[方法]分别采用亚硝酸盐形成法、对苯二胺盐酸盐法及原子吸收分光光度法,测定70例不同期别煤工尘肺患者(Ⅰ、Ⅱ、Ⅲ期)及30例健康对照者的血清CuZn-SOD活性、铜蓝蛋白(CP)活性及Cu的含量。[结果]对照组、Ⅰ期尘肺组、Ⅱ期尘肺组及Ⅲ期尘肺组的血清CuZn-SOD活性分别为(15.96±2.26)、(17.24±2.28)、(19.81±3.01)、(21.63±3.52) NU/ml;血清CP活性分别为(2.26±0.52)、(2.68±0.67)、(2.97±0.79)、(3.36±0.88)U/ml;血清Cu含量分别为(12.68 ±3.87)、(14.96±4.42)、(17.84±5.12)、(19.38±5.89)μmol/L。与对照组相比,各期别尘肺患者血清与CuZn-SOD和CP 活性及其Cu的含量均显著升高。[结论]煤工尘肺的发生发展涉及含Cu抗氧化酶活性的上调,且其活性的改变与Cu含量变化有平行关系。[ Objective ] To explore the changes of serum copper-containing antioxidant enzymes activities including CuZn-SOD, ceruloplasmin （ CP ） and Cu contents in the pathogenesis of coal wocker＇s pneumoconiosis （ CWP ）. [ Methods ] The activities of serum CuZn-SOD, CP and Cu contents in 70 patients with different stage of （ CWP ） （ Ⅰ , Ⅱ , Ⅲ ） and 30 healthy subjects were systematically determined with nitrite forming method, p-phenylene diamine hydrochloride method and atomic absorption spectrophotoassay （ AAS ）, respectively. [ Results ]The activities of serum CuZn-SOD in the control group, Ⅰ stage CWP group,Ⅱ stage CWP group and Ⅲ stage CWP group were （ 15.96 ± 2.26 ）, （ 17.24±2.28 ）, （ 19.81± 3.01 ）, （ 21.63±3.52 ） NU/ml, respectively, the activities of CP were （ 2.26±0.52 ）, （ 2.68±0.67 ）, （ 2.97±0.79 ）, （ 3.36±0.88 ） U/ml, respectively, and the contents of Cu were （ 12.68 ± 3.87 ）, （ 14.96 ± 4.42 ）, （ 17.84 ± 5.12 ）, （ 19.38 ± 5.89 ）μmol/L, respectively. As compared with the control subjects, the activities of serum CP, CuZn-SOD and Cu levels in patients with CWP of different stages significantly increased. [ Conclusion ] The pathogenesis of CWP might be related to the up-regulation of activities of copper-containing enzymes including CP, CuZn-SOD and the changes of these enzymes activities are also closely correlated with the changes of Cu contents.

关 键 词：煤工尘肺 铜蓝蛋白 CUZN-SOD CU 

分 类 号：R135.1[医药卫生—劳动卫生]