卡维地洛对大鼠急性心肌梗死后胶原网络重构的影响  

Experimental study of effect of carvedilol on myocardial collagen network remodeling after acute myocardial infarction in rats

作　　者：刘宏斌[1] 边素艳[1] 杨庭树[1] 王莉[1] 易军[1] 杨霞[1] 

机构地区：[1]解放军总医院心内科实验室,北京100853

出　　处：《中国危重病急救医学》2005年第9期537-540,共4页Chinese Critical Care Medicine

基　　金：全军医药卫生科研基金资助项目(01Z036)

摘　　要：目的研究卡维地洛、厄贝沙坦及二者合用对急性心肌梗死(AMI)大鼠胶原网络重构的影响。方法结扎左前降支建立雄性SD大鼠AMI模型,35只术后24h存活大鼠随机分为阳性对照组(n=8)、卡维地洛组(n=9,10mg·kg-1·d-1)、厄贝沙坦组(n=9,45mg·kg-1·d-1)和卡维地洛(10mg·kg-1·d-1)与厄贝沙坦(45mg·kg-1·d-1)合用组(n=9),另设假手术组(n=8)。治疗组直接灌胃给药干预8周后测定血流动力学参数及心功能。苦味酸天狼猩红染色心脏切片于偏振光显微镜下测量左心室梗死区及非梗死区总胶原容积分数(CVF)及、型CVF。结果AMI大鼠各组间梗死面积差异无显著性(40.02%～44.70%)。与假手术组相比,阳性对照组大鼠左心室舒张末压,左、右心室相对重量,左心室梗死和非梗死区、型CVF及总CVF均显著增加(P<0.05或P<0.01);平均血压、左心室收缩压(LVSP)、左心室内压最大上升和下降速率(±dp/dtmax)及其校正值(±dp/dtmax/LVSP)均显著降低(P<0.05或P<0.01)。与阳性对照组相比,3个治疗组左心室舒张末压,左、右心室相对重量,非梗死区总CVF及、型CVF均显著降低(P<0.05或P<0.01),但±dp/dtmax及±dp/dtmax/LVSP均显著升高(P均<0.01)。结论单用卡维地洛或厄贝沙坦与二者合用均能有效地减少左心室非梗死区胶原沉积,抑制大鼠AMI后的左心室重构,改善血流动力学和左心室功能。Objective To investigate the effects of carvedilol, irbesartan and their combination on myocardial collagen network remodeling after acute myocardial infarction（AMI） in rats. Methods Twentyfour hours after ligating left anterior decending coronary artery, 35 surviving AMI male Sprague - Dawley rats were randomly assigned to control （n=8）, carvedilol （n=9, 10mg·kg^-1·d^-1）, irbesartan （n=9, 45mg·kg^-1·d^-1）, and carvedilol （10mg·kg^-1·d^-1） plus irbesartan （45mg·kg^-1·d^-1, n= 9） groups. Sham operating group was comprised of 8 rats without coronary artery ligation as controls. After 8 weeks of administration of the drug by gastric gavage, hemodynamics and left ventricular function were measured, then the rat hearts were fixed, sectioned, and stained with Sirius red, and pathologically analyzed using polarized light. The total collagen volume density fraction （CVF） and type Ⅰ and Ⅲ CVF in the infarcted and noninfarcted zone （IZ/NIZ） were measured by computer -assisted image analysis system. Results There were no significant differences in myocardial infarction size among the four AMI groups （40. 02%-44.70%, P〉0.05）. Compared with the sham operation group, left ventricular （LV） end diastolic pressure （LVEDP）, left and right ventricular relative weight （LVRW/RVRW）, the total CVF and the CVF of type Ⅰ and Ⅲ in the IZ and NIZ were all significantly higher （P〈0.05 or P〈0.01）, and in contrast, blood pressure, left ventricular systolic pressure （LVSP）, the left ventricular pressure maximal rate of rise and fall （±dp/dt max） and their adjustment by LVSP （±dp/dt max/LVSP） were significantly decreased （P〈0.05 or P〈0.01）. Compared with the control group, LVEDP, LVRW, RVRW, the total CVF and the CVF of type Ⅰ and Ⅲ in the NIZ were all significantly decreased （P〈0.05 or P〈0.01）, while ±dp/dt max and ±dp/dt max/L VSP were all significantly increased （all P〈0.01） in the carvedilol, irbesartan and their combination t

关 键 词：心肌梗死 胶原 心室重构 大鼠 卡维地洛 厄贝沙坦 SD大鼠 胶原沉积 网络重构 急性心肌梗死后 

分 类 号：R542.22[医药卫生—心血管疾病]