实时荧光定量聚合酶链反应技术检测人早孕蜕膜和黄体中期子宫内膜白血病抑制因子的表达  

Real-time polymerase chain reaction for quantitation of leukemia inhibitory factor in early pregnant decidua and mid-luteal phase endometrium

作　　者：任平[1] 武泽[1] 章晓梅[1] 邓波[1] 邵静宜[1] 孟昱时[1] 

机构地区：[1]云南省第一人民医院生殖遗传科,昆明650032

出　　处：《生殖医学杂志》2005年第4期214-216,共3页Journal of Reproductive Medicine

基　　金：云南省自然科学青年基金项目(2001C0036Q)

摘　　要：目的探讨白血病抑制因子(LIF)在人早孕期子宫蜕膜和黄体期子宫内膜的表达情况。方法采用实时荧光定量聚合酶链反应(PCR)技术,对36例正常早孕子宫蜕膜和34例输卵管阻塞妇女黄体中期子宫内膜进行LIF定量检测。结果黄体中期子宫内膜LIF表达量高于早孕期子宫蜕膜组织,表达中位数(M)分别为240.00和46.50 ng/L。两组比较差异有显著性(P<0.01)。结论LIF基因在黄体中期(胚泡着床期)子宫内膜表达量较高,在早孕期子宫蜕膜表达量较低,提示黄体中期和早孕期LIF基因表达量的差异与胚泡着床和妊娠维持的不同生理阶段有关。Objective： To investigate the expression of leukemia inhibitory factor （LIF） in early pregnant decidua and mid-luteal phase endometrium. Methods： Real-time quantitative polymerase chain reaction was performed to determine LIF gene expression in early pregnant decidua and mid-luteal phase endometrium. Result： There were higher LIF levels in mid-luteal phase endometrium （median 240.00 ng/L）, as compared with those in early pregnant decidua （median 46.50 ng/L）. There was a significant difference between two groups（P〈0.01）. Conclusion： Higher LIF gene expression in mid-luteal phase endometrium indicate that LIF is probably involved in initiating blastocyst implantation while the lower expression of LIF in decidua may be related with maintaining early pregnancy.

关 键 词：子宫内膜 白血病抑制因子 早孕期 黄体中期 实时荧光定量聚合酶链反应 

分 类 号：R714.1[医药卫生—妇产科学] R446.1[医药卫生—临床医学]