人急性早幼粒细胞白血病SCID小鼠模型的建立  

Establishment of Human Acute Myeloid Leukemia Model in the SCID Mouse

作　　者：解启莲[1] 甄素芬[1] 李丽红[2] 

机构地区：[1]河北省儿童医院,石家庄050031 [2]首都医科大学附属北京朝阳医院

出　　处：《中国比较医学杂志》2005年第4期203-205,共3页Chinese Journal of Comparative Medicine

摘　　要：目的探讨建立人急性早幼粒细胞白血病小鼠模型的有效方法。方法将4～6周SCID鼠随机分成A、B两组,A组为非照射组、B组为照射组,两组均为每只尾静脉注射2×106HL-60细胞株,每周检测小鼠外周血白细胞计数、外周血涂片人白血病细胞阳性率、病理组织学检测小鼠瘤细胞弥散生长浸润肝、脾等器官情况。结果注射3周后两组外周血白细胞计数两组差异无显著性。但外周血涂片人白血病早幼粒细胞细胞阳性率分别为8.3%、5.4%,B组高于A组(P<0.05)。而病理组织学证实两组小鼠注射瘤细胞28d后均有器官浸润如肝、脾、肾肺等,且B组远较A组广泛而严重。结论照射组和非照射组Scid小鼠静脉2×106HL-60细胞株均能建成人类粒细胞白血病小鼠模型。照射组更能模拟临床白血病累及骨髓和弥漫生长特点,是一个体内研究人类白血病发病机理及实验治疗良好的模型。Objective To explore the effective method of establishing human myeloid leukemia mouse model. Methods 6 to 8-week-old SCID mice were divided randomly into two groups. Group A was unirradiated and group B sublethally irradiated with 200 eGy from a ^6o Co source. All SCID mice were inoculated 2 × 10^6 HL-60 cells via tail vein. Every week after inoculation the peripheral blood white cell count and the positive rates of promyelocytes on smears were examined in two groups. The histology assay was employed for HL-60 cells infiltration in organs （liver, spleen, lung and kidney ）of leukemia-bearing mice. Results Three weeks after inoculation there was no significant difference in peripheral blood cell count between two groups. But the positive rates of promyelocytes on smears were 8.3% and 5.4% , respectively ,with group B higher than group A （ P 〈 0.05）. Histological assay confirmed that all mice of the two groups developed myeloid leukemia after 28 days, and the organs infiltration （ liver, spleen, kidney,etc） in group B was broader and more serious than that in group A. Conclusion The establishment of human myeloid SCID mouse model is available by intravenous inoculation to unirradiatod and sublethally irradiated SCID mouse with （2 × 10^6 ）HL-60 cells. Nevertheless sublethally irradiated SCID mouse model are more mimic to characterization of involved marrow and widespread for leukemia in clinic. This model is a useful tool for studying pathogenesis and experimental treatment of human leukemia.

关 键 词：白血病 模型 动物 小鼠 SCID HL-60细胞 

分 类 号：Q95-33[生物学—动物学]