近交系小鼠H-2抗原单克隆抗体的对比研究  

Comparative Research of Monoclonal Antibodies against H-2 Antigens of Inbred Strain Mouse

作　　者：马丽颖[1] 郑继奎[2] 谭萍萍[3] 刘双环[1] 岳秉飞[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]哈尔滨医科大学实验动物部 [3]中国科学院遗传与发育研究所

出　　处：《中国比较医学杂志》2005年第4期210-212,共3页Chinese Journal of Comparative Medicine

摘　　要：目的对比研究进口抗近交系小鼠H-2抗原单克隆抗体和国产抗近交系小鼠H2单克隆抗体的免疫反应是否一致,以尽快推广国产抗体的使用。方法利用微量细胞毒法,通过倒置显微镜观察死亡细胞(阳性细胞)的百分比来判定国外抗近交系小鼠H2抗原单克隆抗体和国产抗近交系小鼠H2单克隆抗体的免疫反应结果。结果进口抗体:H-2Db(271113)、H-2Dd(3458S)、H-2Dk(1555.3)、H-2Kk(163.22.4)与相应品系抗原反应,脾细胞死亡数达到60%以上。国产抗体:H-2Db(2)、H-2Dd(4)、H-2Dk(32)、H-2Kb(33)、H-2Kd(31)、H2-Kk(23)分别与相应品系抗原反应,致脾细胞死亡数达60%以上。国产和进口单抗最终免疫反应结果一致。结论国产抗近交系小鼠H-2抗原单克隆抗体可以用于检测小鼠H-2D区和H-2K区。Objective To compare the reactivity of imported and domestic monoclonal antibodies against H-2 antigens of inbred strain mouse. Methods The percentage of quantity of obituary cells （positive cells ） was acquired by using mieroeytotoxieity test. Result The imported antibodies H-2D^b（27-11-13）, H-2D^d（34-5-8S）, H-2D^k（15-5-5.3）, H-2K^k（16-3.22.4） reacted to the homologous antigens and the death rate of the spleen cells was more than 60% . The domestic antibodies H-2D^b （2）, H-2D^d （4）, H-2D^k （32）, H-2K^b （33）, H-2K^d （31）, H-2K^k （23） induced death rate of spleen cells was also over 60%. The reaction of both imported and domestic monoelonal antibodies against H-2 antigens of inbred strain mouse was in consistency. Conclusion The domestic monoclonal antibodies against H-2 antigens of inbred strain mouse can also be applied to monitor the mouse＇ s H-2D and H-2K in mice.

关 键 词：抗体 单克隆 免疫遗传 监测 

分 类 号：Q953[生物学—动物学]