乙酰短杆菌α-乙酰乳酸脱羧酶基因的克隆及其表达  

Cloning and Expression of the α-acetolactate Decarboxylase Gene from Brevibacterium Actylium in E. coli

作　　者：沈亮[1] 

机构地区：[1]德州学院生物系,山东德州253023

出　　处：《德州学院学报》2005年第4期5-7,共3页Journal of Dezhou University

摘　　要：用PCR方法扩增乙酰短杆菌α-乙酰乳酸脱羧酶基因(ALDC),得到0.78 kb的DNA片段,扩增片段重组到表达载体pQE-9中,在大肠杆菌中得到高效表达,酶活性可达100 U/mL发酵液.The ALDC gene was cloned by PCR using Brevibacterium actyliurn chromosome DNA as template and got 0. 78 kb DNA fragment. The fragment was inserted directionally into a prokaryotic expression vector pQE-9 with high efficiency. The ALDC gene was overexpressed in E. coli JM109, and the production of ALDC was over 100 U/mL.

关 键 词：乙酰短杆菌 α-乙酰乳酸脱羧酶基因 大肠杆菌 高效表达 

分 类 号：Q935[生物学—微生物学]