Fine mapping and marker-assisted selection (MAS) of a low glutelin content gene in rice  

作　　者：Yi Hua WANG Shi Jia LIU Su Lan JI Wen Wei ZHANG Chun Ming WANG Ling JIANG Jian Min WAN 

机构地区：[1]The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University Jiangsu Re-search Center of Plant Engineering, Nanjing 210095, China [2]Crop Science Research Institute, Chinese Academy of Agricuhural Science, Beijing 100081, China

出　　处：《Cell Research》2005年第8期622-630,共9页细胞研究（英文版）

基　　金：supported by the grants from Hi-Tech Research and Development Program of China("863"Program,No.2003AA222131,2003AA207020);the National Natural Science Foundation of China(No.30170570);Special Program for gene-transfering(No.JY03-B-07,JY03-A-07-02)

摘　　要：Rice with low glutelin content is suitable as functional food for patients affected with diabetes and kidney failure. The fine mapping of the gene(s) responsible for low glutelin content will provide information regarding the distribution of glutelin related genes in rice genome and will generate markers for the selection of low glutelin rice varieties. Following an SDS-PAGE screen of rice germplasm from Taihu Valley of China, Japonica selection W3660 is identified to be a novel mutant characterized with low glutelin content. For fine mapping the mutant gene for low glutelin content, F2 and F3 populations were derived from a cross between W3660 and Jingrennuo. SDS-PAGE analysis of the total endosperm protein showed that the low glutelin content trait was controlled by a single dominant nuclear gene. Genetic mapping, using SSRs, located this gene to chromosome 2, in the region between SSR2-001/SSR2-004 and RM1358. The dis- tances of the two markers to the target gene were 1.1 cM and 3.8 cM respectively. By semi-quantitative RT-PCR analysis, the transcripts of GluB4/GluB5 genes located within the region do not change. However, GluB5 gene located proximal to SSR2-001/SSR2-004 was specifically reduced. SSR profiles of seven Japonica varieties were compared with that of W3660 for loci in the relevant genetic region. The markers SSR2-004 and RM1358 were used for marker- assisted selection. The selection efficiencies of SSR2-004 and RM1358 were 96.8% and 92.7% respectively. This provides a standard starting point for the breeding of low glutelin content rice varieties in China.

关 键 词：fine mapping low glutelin content marker-assisted selection mutant rice. 

分 类 号：S511[农业科学—作物学]