Identification of Common Species of Dermatophytes by PCR-RFLP  

作　　者：何甘霖 李家文 丁娟 谭志健 

机构地区：[1]epartment of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2005年第4期458-460,共3页华中科技大学学报（医学英德文版）

摘　　要：To establish a simple, sensitive and effective technique for the identification of six common dermatopbytes, polymerase chain reaction （PCR） and PCR-restriction fragment length polymorpbism （RFLP） targeting Topoisomerase Ⅱ gene were used. The DNA of 6 common dermatopbytes was amplified by primer dPsD1 and then primers dPsD2. The products generated by dPsD2 were digested with restriction enzyme Hinc Ⅱ and Hinf Ⅰ separately. A DNA fragment of about 3390 bp was amplified by using primer dPsD1 from the genomic DNA of each dermatopbyte species. The product of dPsD2 was 2380 bp and the restriction profiles of Hinc Ⅱ and Hinf Ⅰ were between 58-1670 bp. By using PCR-RFLP, all of the 6 dermatopbytoses were diagnosed to species level and no obvious difference identification between Hinc Ⅱ and Hinf Ⅰ. It is concluded that the PCR-RFLP identification of dermatopbytes by Hinc Ⅱ or Hinf Ⅰ is efficient and rapid in clinical practice.To establish a simple, sensitive and effective technique for the identification of six common dermatopbytes, polymerase chain reaction （PCR） and PCR-restriction fragment length polymorpbism （RFLP） targeting Topoisomerase Ⅱ gene were used. The DNA of 6 common dermatopbytes was amplified by primer dPsD1 and then primers dPsD2. The products generated by dPsD2 were digested with restriction enzyme Hinc Ⅱ and Hinf Ⅰ separately. A DNA fragment of about 3390 bp was amplified by using primer dPsD1 from the genomic DNA of each dermatopbyte species. The product of dPsD2 was 2380 bp and the restriction profiles of Hinc Ⅱ and Hinf Ⅰ were between 58-1670 bp. By using PCR-RFLP, all of the 6 dermatopbytoses were diagnosed to species level and no obvious difference identification between Hinc Ⅱ and Hinf Ⅰ. It is concluded that the PCR-RFLP identification of dermatopbytes by Hinc Ⅱ or Hinf Ⅰ is efficient and rapid in clinical practice.

关 键 词：DERMATOPHYTE PCR RFLP 

分 类 号：R446.5[医药卫生—诊断学]