葡萄籽原花青素提取物对大鼠乙酸性结肠炎的保护作用  

Protective effects of grape seed proanthocynidins extract on acetic acid-induced colitis in rats

作　　者：杨孝来[1] 吴勇杰[1] 葛斌[2] 王莉[1] 李文广[1] 高明堂[1] 

机构地区：[1]兰州大学药理学教研室 [2]甘肃省人民医院药剂科

出　　处：《中国临床药理学与治疗学》2005年第8期903-908,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：甘肃省自然科学基金项目(№ZS031-A25-058-E)

摘　　要：目的:研究葡萄籽原花青素提取物(GSPE)对大鼠乙酸性结肠炎的治疗作用。方法:制备大鼠乙酸性结肠炎模型,分别设正常对照组、模型对照组、阳性对照组和GSPE低、中、高剂量组,共6组。每天给药1次,共7d。于给药d8时进行大鼠病变结肠大体及组织学评分;用邻联茴香胺法检测结肠组织中MPO活性以考察中性白细胞浸润程度。结果:实验1周时,模型对照组大鼠体重明显下降,结肠湿重明显增加,和模型对照组比较,GSPE低、中剂量组体重下降程度减轻(P<0.05),GSPE高剂量组体重呈增加趋势(P<0.01)。GSPE各剂量组结肠湿重增加幅度较模型对照组明显降低(P<0.05)。大体观察,模型对照组结肠粘膜大面积溃疡、坏死,和模型对照组比较,GSPE各剂量组结肠粘膜溃疡面积缩小,粘膜修复明显。光镜下观察,模型对照组结肠粘膜组织内可见大量炎性渗出物和组织坏死;GSPE各剂量组溃疡面可见多量再生上皮和新生腺体。模型对照组结肠粘膜组织内MPO含量较正常对照组明显升高(P<0.01),GSPE各剂量组MPO活性较模型对照组明显降低(P<0.05)。结论:GSPE对大鼠乙酸性结肠炎有明显的治疗作用,且呈剂量依赖性。AIM： To study the therapeutic effects of grape seed proanthocynidins extract（GSPE）on acetic acid -induced colitis in rats. METHODS： The rat colitis was induced by intracolonic enema with 10% acetic acid. Animals were divided into six groups： normal group, model group, prednisone treated group, GSPE low, medium or high dose treated group intragastrically, and GSPE or prednisone was given one time a day for seven days. The macroscopic and microscopic colon damage was scored on eighth day. The myeloperoxidase activity in colonic tissue, reflecting neutrophil infiltration was assayed by O-dianisidine method. RESULTS： The average body weight of rats obviously decreased and wet weight of damaged colon seriously increased in model group. Compared with the model group, the suppression of average body weight was obviously improved in rats treated with low or medium dose of GSPE （ P 〈 0.05） and in the contrast, the average body weight of rats treated with high dose of GSPE showed a rise trend （ P 〈 0.01 ）. The increase ofwet weight of the colon was also inhibited by different doses of GSPE （ P 〈 0.05）. It was observed that there were large area ulcers and necrosis on mucous membrane of colon of rats in model group. Compared with the model group, the ulcerative area of colon mucous membrane was reduced and the mucous membrane was repaired obviously in rats treated with different doses of GSPE. Under the microscope, the large amount of inflammation exudation and necrotic tissue in colon sample was seen in model group, but the much more regenerated epithelium and gland body was observed in GSPE treated group. The resuits showed that the MPO activity was higher in model group than that in normal group. Compared with the model group, MPO activity in colon tissue decreased （P 〈 0.01）. CONCLUSION： GSPE has beneficial therapeutic effect on acetic acid-induced colitis of rats in a dosedependen way.

关 键 词：葡萄籽 原花青素 大鼠 结肠炎 抗炎 

分 类 号：R965.1[医药卫生—药理学]