异氟醚预处理对大鼠脑缺血/再灌注损伤细胞内游离钙的影响  

Effects of isoflurane pretreatment on changes of intracellular calcium in cerebral ischemic/reperfusion injury in rats

作　　者：刘艳红[1] 熊利泽[2] 王强[2] 张宏[1] 

机构地区：[1]解放军总医院麻醉科,北京100853 [2]第四军医大学西京医院麻醉科

出　　处：《中国临床药理学与治疗学》2005年第8期909-912,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(№30170907)

摘　　要：目的:观察异氟醚对缺血再灌注大鼠活体脑片细胞内游离钙的影响,探讨异氟醚脑保护作用的机制。方法:采用线栓法阻闭大鼠大脑中动脉造成局灶性脑缺血,并于缺血2h后拔出栓线给予再灌注。利用细胞内钙离子荧光探针Fluo-3/AM在激光共聚焦显微镜下观察再灌注2h后大鼠脑片细胞内荧光强度。比较异氟醚预处理1h对缺血再灌注大鼠活体脑片额顶叶皮质及纹状体区域Ca2+浓度的影响。结果:缺血再灌注损伤大鼠缺血侧脑片皮层及纹状体Ca2+荧光强度明显升高。脑缺血前给予异氟醚预处理1h可抑制缺血侧大脑皮层细胞内Ca2+含量的增加,对纹状体细胞内Ca2+含量的影响无统计学差异。结论:缺血前给予异氟醚预处理可有效减轻额顶叶皮层细胞内的钙超载,对纹状体区域脑细胞内游离钙水平影响较小,提示异氟醚的脑保护作用在不同脑区存在差异。AIM： To investigate the changes of intracellular calcium concentration in vital brain slices after pretreatment with isoflurane in cerebral ischemic/reperfusion rats. METHODS： Focal cerebral ischemia was induced by inserting a suture into the middle cerebral artery of rats. Reperfusion was accomplished by withdrawing the suture after 2 hours＇ ischemia. The changes of intracellular calcium in vital brain slices were determined by fluorescent Ca^2＋-indicator fluo-3/AM. The effects of isoflurane pretreatment were evaluated before cerebral isehemia on the intracellular calcium of cortex and striatum. RESULTS： Intracellular calcium concentration increased significantly both in the cortex and the striatum of the brain after cerebral ischemia/reperfusion, lsoflurane pretreatment given before cerebral ischemia inhibited the elevation of Ca^2＋ in the cortex but not in the stfiatum induced by ischemia/reperfusion injury. CONCLUSION： Isoflurane can inhibit Ca^2＋ overloading in cortex induced by cerebral ischemia/ reperfusion, which may be one of the mechanisms of isoflurane neumpmtection.

关 键 词：异氟醚 预处理 钙 脑缺血 激光共聚焦显微镜 

分 类 号：R966[医药卫生—药理学] R971.2[医药卫生—药学]