二化螟对杀虫单抗性现实遗传力与遗传方式  

Realized Heritability and Inheritance Mode of Monosultap Resistance in Chilo suppressalis(Lepidoptera: Pyralidae)

作　　者：曹明章[1] 沈晋良[1] 

机构地区：[1]南京农业大学植物保护学院农药系

出　　处：《中国水稻科学》2005年第5期447-452,共6页Chinese Journal of Rice Science

基　　金：江苏省十五科技攻关资助项目(BE2001354)

摘　　要：以2001年田间采集的温州二化螟为起始种群,在室内饲养24代期间,用点滴法处理4龄幼虫进行杀虫单筛选19代,得到抗性品系wZ-R;与敏感基线相比,WZ-R品系对杀虫单抗性达774.5倍,比筛选前上升25.0倍;根据前期连续6代(F3～F8)筛选估计的抗性现实遗传力(h2,0.230)显著低于根据后期连续5代(F19～F23)筛选的h2(1.205),而中间不连续筛选(F9～F18,10代中有4代未筛选)的h2最小(0.024).用毒力回归线法对WZ-R品系进行抗性遗传分析表明,杀虫单抗性为不完全显性(正反交显性度分别为0.56和0.63)、常染色体遗传,抗性不属于单个主基因控制.A colony of the striped stem borer, Chilo suppressalis, collected from Wenzhou, Zhejiang Province, was further selected in laboratory with monosultap using topical application method. After selection with monosultap for 19 generations in 24 generations of laboratory culture, resistance to monosultap in the selected strain （WZ-R） increased by 25.0-fold, and 774.5-fold compared with that of the pre-selected and the susceptible baseline of HLJ strain, respectively. The realized heritability （hz ） estimated from early selection stage （0. 230 for F3 -F8, consecutive selection for 6 generations） was significantly smaller than that from later stage （ 1. 205 for F19 -F23, consecutive selection for 5 generations）, while the hz estimated from middle inconsecutive selection stage （F9 -F18, during which there were a total of 4 generations without selection） was the smallest （0. 024）. Inheritance of monosultap resistance in WZ-R strain, studied by log doseprobit （LD-p） analysis for progenies of reciprocal crosses, two backcrosses and a F1 self-cross indicated that resistance to monosultap was inherited autosomaly in an incompletely dominant manner, with a degree of dominance for reciprocal cross F1 s being 0. 56 and 0. 63, respectively, and monosultap resistance in WZ-R appeared to be controlled by two or more genes.

关 键 词：二化螟 抗性 现实遗传力 遗传 杀虫单 

分 类 号：S481.4[农业科学—农药学]