甲基汞急性暴露下大鼠脑c-fos mRNA表达  

Effects of methylmarcury acute exposure on c-fos mRNA expression in brain of rat

作　　者：杨义晨[1] 程金平[1] 胡卫萱[1] 王文华[1] 吴旦[1] 贾金平[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240

出　　处：《中国公共卫生》2005年第9期1074-1075,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目(20177013);上海交通大学985工程资助项目(113516001)

摘　　要：目的探讨即刻早期基因c fos在甲基汞神经毒性分子机制中的作用。方法应用RT PCR方法研究大鼠注射甲基汞急性暴露后,中枢神经系统c fosmRNA表达。结果甲基汞在剂量为0.05mg/(kg·d)暴露60min和剂量为0.5mg/(kg·d)暴露20min就能够显著诱导大鼠脑即刻早期基因c fos表达;c fosmRNA表达量与汞的浓度没有明显正比关系。结论甲基汞能够显著诱导大鼠脑c fosmRNA表达,c fosmRNA表达变化在甲基汞神经毒性分子机制中具有重要作用,即刻早期基因c fos参与了甲基汞对中枢神经系统损害的毒性过程。Objective To study the role that immediate early expression gene（IEG） c-fos played in the neurotoxic mechanism, and the effect of methylmarcury（MMC） on c-fos mRNA expression were investigated. Methods RT-PCR was used for c-fos mRNA expression after MMC acute exposure in rat＇ s brain. The control group was physiological saline of 0.9%, the concentration of exposure groups were 0.05, 0.5 and 5 mg/kg respectively, the sampling time were 20, 60,240 and 1440 min. Results The 0.05 mg/kg group induced the expression of c-fos obviously in rat＇s brain after exposed for 60min while the 0.5 mg/kg group induced the expression of c-fos clearly in rat＇s brain after exposed for 20min, and the expressive quanti- ty of c-fos mRNA did not have direct ratio with the concentration of mercury. Conclusion The expression of c-fos mRNA played an important part in the neurotoxic mechanism of MMC; IEG c-fos took part in the process that MMC impaired the nerve system.

关 键 词：甲基汞 神经毒性 基因表达 C-FOS mRNA 基因c-fos MRNA表达量 急性暴露 大鼠脑 中枢神经系统损害 

分 类 号：R338.64[医药卫生—人体生理学] R259.752[医药卫生—基础医学]