侵染观赏南瓜的黄瓜绿斑驳花叶病毒的初步鉴定  

Preliminary identification of a Cucumber green mottle mosaic virus infecting pumpkin.

作　　者：秦碧霞[1] 蔡健和[1] 刘志明[1] 陈永惠[1] 朱桂宁[1] 黄福新[1] 

机构地区：[1]广西农科院植保所,南宁530007

出　　处：《植物检疫》2005年第4期198-200,共3页Plant Quarantine

基　　金：广西青年科学基金项目(桂科青0135006);广西新世纪十百千人才工程项目(2001215);广西农科院科技发展基金项目(2002025)

摘　　要：从广西某农业展示中心观赏南瓜上分离到一种直杆状病毒,长约300nm,该病毒分离物P3(NN)在测试的葫芦科作物上表现为系统花叶、褪绿斑及明脉症状,在曼陀罗上为局部褪绿斑,在测试的其它茄科作物及豆科和藜科作物上无任何症状反应。其致死温度为95～100℃。经DACELISA测定,P3(NN)与黄瓜绿斑驳花叶病毒有密切的血清学关系。根据以上结果,初步鉴定P3(NN)为黄瓜绿斑驳花叶病毒(CGMMV)的一个分离物。A virus isolate, P-3 （NN） was obtained from pumpkin planted in a agricuhural demonstration zone in Guangxi. P-3（NN） was found to cause systemic mosaic, vein clearing or systemic chlomtic spot in all the Cucuribitaceae plants tested, cause local chlomtic spot in Datura stramonium and on infection in other Solanaceae, Leguminosae or Chenopodiaceae plants tested. The lethal temperature of the virus was 95℃ to 100℃. Straight tubular particles were observed under electron microscope. The length of most virus particles was close to 300nm. ELISA test indicated that P-3（NN） showed a close relationship with Cucumber green mottle mosaic virus （CGMMV）. All the results suggest that P-3 （NN） is an isolate of CGMMV.

关 键 词：观赏南瓜 黄瓜绿斑驳花叶病毒 病毒鉴定 花叶病毒 初步鉴定 黄瓜 葫芦科作物 侵染 分离物 杆状病毒 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]