套式聚合酶链反应技术检测母乳中乙肝病毒  

Detecting Hepatitis B Virus Deoxyribonucleic Acid Gene in Breast Milk by Nested Polymerase Chain Reaction

作　　者：虞小梅[1] 胡微虹 杜建明[1] 张茵 

机构地区：[1]宁波市妇女儿童医院,浙江宁波315012 [2]椒江市人民医院,浙江椒江317700

出　　处：《实用儿科临床杂志》2005年第9期866-867,共2页Journal of Applied Clinical Pediatrics

摘　　要：目的采用从母乳标本中快速检测乙型肝炎(乙肝)病毒(HBV-DNA)基因片段方法,对乙型肝炎病毒表面抗原(HBsAg)阳性母亲产后乳汁是否携带HBV做出早期诊断。方法收集187例HBsAg阳性母亲乳汁,采用套式聚合酶链反应(nested PCR)扩增乳汁标本中HBV-N基因相关片段。结果套式PCR可检测到乳汁中HBV-DNA。检测病毒阳性6例,阳性率3.2%。结论套式PCR检测乳汁中的HBV-N基因可作为HBsAg阳性母亲能否母乳喂养的实验室依据之一。Objective With the method of detecting hepatitis B virus deoxyribonucleic acid（HBV DNA） gene in breast milk, in ordered to diagnose whether there is HBV in breast milk in those mothers whose HBsAg are positive. Methods Collected breast milk from 187 mothers whose HBsAg were positive, and complified HBV N gene in breast milk by Nested - Polymerase chain reaction （Nested - PCR）. Results Nested PCR could detect HBV DNA in breast milk, and the positive rate was 3.2 %. Conclusion The method of detecting HP, V -N gene in breast milk by Nested-PCR can detect HBV - DNA in breast milk, and it can be an laboratory evidence for whether breast feeding or not.

关 键 词：肝炎病毒 乙型 DNA 母乳 聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学]