河南省结核分枝杆菌IS6110DNA指纹图谱特征分析  

Analysis of DNA fingerprint′s features of Mycobacterium Tuberculosis IS6110 in Henan province

作　　者：闫国蕊[1] 程豪为 李为民[3] 腊胜明[1] 

机构地区：[1]河南省疾病预防控制中心,郑州450008 [2]河南省医学科学研究所,郑州450052 [3]北京结核病胸部肿瘤研究所,北京101149

出　　处：《中国卫生检验杂志》2005年第9期1033-1034,共2页Chinese Journal of Health Laboratory Technology

基　　金：河南省科技攻关资助项目(0124170504)

摘　　要：目的:从分子流行病学角度探讨河南省结核分支杆菌的分布特征。方法:样本的获得采用全省范围等比例分层整群随机抽样;构建以IS6110 RFLP DNA指纹方法和以DR为基础的Spoligotyp ing DNA指纹方法;采用Gel compare4.1软件对DNA指纹图谱进行聚类(c luster)分析。结果:共获得66株可进行IS6110 RFLP DNA指纹分析的结核分支杆菌;其中59.09%(39/66)用Spoligotyp ing DNA指纹方法鉴定为“北京基因型”菌株。结论:北京基因型结核分支杆菌在河南省呈较高水平的流行。Objective：To explore the epidemical distribution characteristics of Mycobacterium tuberculosis isolated from Henan province. Methods：The M. tuberculosis strains were gained by multi -stratified grouping random sampling method from the tuberculosis epidemiology survey in Henan and analyzed by IS6110- based RFLP and DR -based Spoligotyping DNA fingerprinting. These fingerprinting patterns were clustering by Gel compare 4. 1 software. Results：Thirty - nine of 66 M. tuberculosis strains from this survey were determined to be “ Beijing Genotype” M. tuberculosis strain by DNA fingerprinting analysis. Conclusion： M. tuberculosis strains of Beijing Genotype are prevailing in Henan at present.

关 键 词：结核分支杆菌 DNA指纹 SPOLIGOTYPING 聚类分析 

分 类 号：R378.911[医药卫生—病原生物学]