海南省东方市恶性疟原虫对双氢青蒿素复方组分敏感性的体外检测  

In-vitro Sensitivity of Plasmodium falciparum to the Components of Dihydroartemisinin Compounds in Dongfang City of Hainan Province

作　　者：刘莱莱[1] 陈沛泉[1] 欧凤珍[1] 符崇威[1] 

机构地区：[1]广州中医药大学热带医学研究所,广州510405

出　　处：《广州中医药大学学报》2005年第5期377-378,382,共3页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家中医药管理局重点课题(编号:95B128)

摘　　要：[目的]检测海南省东方市恶性疟原虫对双氢青蒿素复方组分双氢青蒿素(DHA)和磷酸哌喹(PQ)的敏感性。[方法]采用世界卫生组织(WHO)标准的体外微量法。[结果]2001年和2004年分别对61例和28例患者检测DHA敏感性,均敏感,IC50分别为6.84 nmol/L和5.67 nmol/L,完全抑制裂殖体形成的平均药物浓度分别为35 nmol/L和39 nmol/L;分别对75例和29例患者检测PQ敏感性,抗性率分别为22.67%和17.24%,IC50分别为274nmol/L和317nmol/L,完全抑制裂殖体形成的平均药物浓度分别为1 220nmol/L和1 269 nmol/L。[结论]当地恶性疟原虫对DHA是敏感的,对PQ的敏感性有所恢复。[ Objective] To examine the sensitivity of P. falciparum to the components of dihydroartemisinin （DHA） and piperaquine （PQ） in Dihydroartemisinin Compounds. [Methods] WHO （World Health Organization） standard microtest and in-vitro test were used. [Results] Survey in the year of 2001 for 61 cases and in 2004 for 28 cases showed that the DHA-resistant rate of P. falciparum in all cases was zero with inhibitory concentration of 50% （ICs0） being 6.84 nmol/L and 5.67 nmol/L, and the mean concentration for complete inhibition of schizont formation was 35 nmol/L and 39 nmol/L, respectively. Survey in the year of 2001 for 75 cases and in 2004 for 29 cases showed that PQ-resistant rate of P. falciparum was 22.67% and 17.24% with ICs0 being 274 nmol/L and 317 nmol/L, and the mean concentration for complete inhibition of schizont formation was 1 220 nmol/L and 1 269 nmol/L, respectively. [ Conclusion] No DHA-resistance exists and PQ-resistance decreases for the treatment of P. falciparum in Dongfang city of Hainan province.

关 键 词：疟疾 恶性/中药疗法 疟原虫 恶性 双氢青蒿素复方/治疗作用 敏感性与特异性 

分 类 号：R285.5[医药卫生—中药学]