小四五颗粒对糖尿病大鼠肾脏肾素-血管紧张素系统的影响  

Effect of Xiao Si Wu Granules on Renal Renin-angiotensin System in Diabetic Rats

作　　者：雷作熹[1] 罗仁[1] 赵晓山[1] 董晓蕾[1] 

机构地区：[1]南方医科大学南方医院中医科,广州510515

出　　处：《广州中医药大学学报》2005年第5期389-392,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省自然科学基金项目(编号:013040);广东省中医药管理局科研基金项目(编号:101004)

摘　　要：[目的]观察中药复方小四五颗粒对糖尿病(DM)模型大鼠肾脏肾素-血管紧张素系统(RAS)的影响。[方法] Wistar大鼠24只,随机分为正常组、模型组和小四五颗粒组(中药组);除正常组外,其他大鼠均腹腔一次性注射链脲佐菌素(STZ)65 mg/kg复制DM模型;中药组灌胃小四五颗粒1.8 g·kg-1·d-1,其他2组给予等客积生理盐水,连续12周;采用放射免疫法检测肾脏血管紧张素Ⅱ(AngⅡ)浓度,紫外分光光度法检测大鼠肾皮质血管紧张素转化酶(ACE)活性,逆转录聚合酶链反应(RT-PCR)法检测大鼠肾皮质血管紧张素Ⅱ1型受体(AT1)mRNA含量。[结果]模型组肾脏AngⅡ浓度、肾皮质ACE活性均升高,肾皮质AT1 mRNA含量降低,与正常组比较均有显著性差异(P<0.01);中药组肾脏AngⅡ浓度、肾皮质ACE活性均降低,与模型组比较均具有显著性差异(P<0.05),AT1 mRNA含量与模型组比较无显著性差异。[结论]小四五颗粒治疗DM肾损伤的机制可能与其对DM肾脏内RAS活化具有一定调节作用有关。[ Objective] To investigate the effect of Xiao Si Wu Granules （XSWG）, a preparation composed of Xiao Chaihu Decoction, Siwu Decoction and Wuling Powder, on renal renin-angiotensin system （RAS） in diabetic rats. [Methods] Twenty-four Wistar rats were randomly divided into the normal, control and XSWG groups. Except the normal group, the rats in other groups were given one-dose intraperitoneal injection of streptozotocin （STZ） 65 mg/kg to induce diabetes mellitus （DM）. After the establishment of diabetic models, XSWG group was treated with XSWG 1.8 g·kg^-1·d^-1 .and the other two groups with the same dose of saline for 12 weeks. After treatment, the intrarenal angiotensin Ⅱ （Ang Ⅱ ） concentration was detected with radioimmunoassay method, renocortical antiotensin-converting enzyme （ACE） activity with ultraviolet spectrophotometry and renocortical angiotensin Ⅱ receptor type 1 （AT1） mRNA level with reverse transcriptase polymerase chain roaction （RT-PCR）, [ Results] Ang Ⅱ concentration and renocortieal ACE activity were increased, and renocortical AT1 mRNA level was decreased in the model group, the difference being significant as compared with the normal group （ P 〈 0.01 ）. Ang Ⅱ concentration and renocortical ACE activity were decreased in XSWG group, the difference being significant as compared with the model group （ P 〈 0.05 ）. The difference of AT1 mRNA level was insignificant between XSWG group and the model group. [ Conclusion] The possible therapeutic mechanism of XSWG for DM is related to the regulation of activity of renal RAS in DM.

关 键 词：小四五颗粒/药理学 糖尿病肾病/中药疗法 肾素—血管紧张素系统 疾病模型 动物 大蛊 

分 类 号：R285.5[医药卫生—中药学]