银杏16个主要栽培品种的分子鉴别  

Molecular Identification of 16 Main Cultivars of Ginkgo biloba Linn.

作　　者：谭晓风 冯晓黎[1] 胡芳名 杨伟[1] 

机构地区：[1]中南林学院经济林育种与栽培国家林业局重点实验室

出　　处：《中南林学院学报》2005年第4期35-39,共5页Journal of Central South Forestry University

基　　金：国家科技基础条件平台工作项目"林木种质资源标准化整理;整合及共享试点"(编号:2004BKA30400);国家林业局重点项目"银杏香榧无性系及黄山松与黑松种子的分子鉴别"(编号:2001-29)

摘　　要：以16个银杏栽培品种的单倍体胚乳DNA为材料,用筛选出了高效引物进行RAPD分析,确定了各品种的标记基因型.在此基础上,可利用单倍体种子胚乳或二倍体叶片等材料实现对银杏无性系品种的有效分子鉴别,为银杏的良种鉴别、保护和登录提供了科学依据.The haploidic endosperm DNA samples of sixteen main cultivars of Ginkgo biloba Linn. were analyzed with RAPI） technology by using selected primers with distinct polymorphism, and the marker genotypes of each cultlvar were confirmed. Based on this result, molecular identification of clones （cultivars） of GiJUego biloha Linn. can be effectively performed by using haploidic seed endosperm or diploid foliage as experimental materials, providing a scientific foundation for the identification, protection and registration of Ginkgo improved varieties.

关 键 词：银杏 品种 RAPD 分子鉴别 

分 类 号：S792.95[农业科学—林木遗传育种] Q523[农业科学—林学]