硒代蛋氨酸与亚硒酸钠对LLC-PK_1细胞DNA合成影响的比较  

Comparision between the effects of selenomethionine and sodium selenite on DNA synthesis of pig kidney epithelial cell line (LLC-PK_1)

作　　者：彭双清[1] 刘世杰[2] 

机构地区：[1]军事医学科学院毒物药物研究所 [2]北京医科大学公共卫生学院,北京100083

出　　处：《卫生研究》1996年第1期50-52,共3页Journal of Hygiene Research

基　　金：国家自然科学基金

摘　　要：采用＾３Ｈ－ＴｄＲ掺入实验观察了Ｄ，Ｌ－硒代蛋氨酸与亚硒酸钠对猪肾近曲小管上皮细胞（ＬＬＣ－ＰＫ１）ＤＮＡ合成代谢的影响，结果显示，两种硒化合和的对ＤＮＡ的合成均呈双相效应，在硒浓度为０．５～５０μｍｏｌ／Ｌ时，能促进ＤＮＡ合成，并且Ｄ，Ｌ－硒代蛋氨酸的作用比亚硒酸钠更为有效；随硒浓度的增高，两种硒化合物均呈现对ＬＬＣ－ＰＫ１细胞ＤＮＡ合成的抑制作用，亚硒酸钠的毒性作用明显大于Ｄ，Ｌ－硒代蛋氨酸，The effects of selenomethionine(SeMet) and sodium selenite(Na_2SeO_3) on DNA synthesis were studied in pig kidney epithelial cell line (LLC-PK_1). Results revealed a biphasic effects of both Na_2SeO_3 and SeMet on DNA synthesis. At concentrations between 0.5 and 50μmol/L, both of selenium compounds stimulated cell DNA synthesis and SeMet was more effective. At concentrations of 200μmol/L and above they inhibited cell DNA synthesis, and the toxicity of Na_2SeO_3 was greater than SeMet.

关 键 词：硒代蛋氨酸 亚硒酸钠 肾小管 上皮细胞 DNA 

分 类 号：R977.5[医药卫生—药品] Q585[医药卫生—药学]