低温预处理对刺槐种子抗盐萌发的影响  

Effects of pre-chilling on germination of Robinia pseudoacacia under salt stress

作　　者：曹帮华[1] 翟明普[1] 吴丽云[2] 

机构地区：[1]北京林业大学资源与环境学院 [2]山东农业大学林学院

出　　处：《北京林业大学学报》2005年第4期39-42,共4页Journal of Beijing Forestry University

基　　金：国家科技部农业科技成果转化项目(02EFN216700794);"十五"北京市重大科技攻关项目(H020720110190).

摘　　要：先将刺槐种子在始温80℃下浸泡24h,取吸胀的种子用低温3～5℃处理5、10、15d,置于不同盐分梯度下做发芽试验,观察种子的萌发能力.研究结果表明:在低于0.5%盐浓度下,低温处理5d能显著提高种子的发芽率和发芽势,发芽指数与活力指数明显高于对照;低温处理10d次之;低温处理15d对种子有伤害作用.低温处理组可溶性蛋白含量、超氧物岐化酶(SOD)的活性普遍提高;0.2%盐浓度下处理组过氧化物酶(POD)高于对照组,0.2%以上处理组显著低于对照;各种盐浓度下低温处理组过氧化氢酶(CAT)的活性均显著高于对照.该研究结果表明种子萌发前经5d低温(3～5℃)预处理能有效提高盐胁迫下刺槐种子的萌发能力.The imbibed black locust seeds were treated with pre-chilling （3- 5℃） for different days （5,10 and 15 d） and their germination capacity under different concentrations of NaCl （0,0.1%, 0.2%, 0.3 %, 0.4% and 0.5%）was analyzed. Results show that the germination rate and germination energy of black locust seeds treated with NaCl for 5 d increased significantly, and their germination and vigor index were also higher than those of control if NaCl concentration was not more than 0.5 % ; followed by the seeds treated with pre-chilling for 10 d; the seeds would be injured with a pre-chilling time more than 15 d. During the gemination the activities of superoxide dismutase （SOD） and catalase （CAT） of treated seeds all increased; the activity of peroxidase （POD） was higher （NaCl concentration less than 0.2%） or less （NaCl concentration more than 0.2%） than those of control. These indicate that pre-chilling treatment could improve both the salt resistance and the seedling growth of black locust seeds during the germination.

关 键 词：刺槐种子 低温预处理 抗盐 萌发 

分 类 号：S792.26[农业科学—林木遗传育种]