白花前胡甲素对豚鼠单一心室肌细胞钙电流的频率依赖性阻断作用  

Frequency-dependent block of calcium current by (±)- praeruptorin A in single ventricular cell of guinea pig

作　　者：郝丽英[1] 李金鸣[1] 常天辉[1] 孙晓东[1] 王玉萍[1] 张克义[1] 

机构地区：[1]中国医科大学基础医学部药理教研室

出　　处：《中国药理学与毒理学杂志》1996年第1期17-20,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：the;Science;Foundation;of;Ministry;of;Public;Health

摘　　要：本文利用全细胞膜片钳制技术，观察了白花前胡有效成分Ｐｒａ－Ａ对豚鼠单一心室肌细胞钙电流的影响．结果表明：１，１０，１００μｍｏｌ·Ｌ－１Ｐｒａ－Ａ可使钙电流峰值变小，随浓度增加，作用加强；１０μｍｏｌ·Ｌ－１Ｐｒａ－Ａ对钙电流的抑制具使用依赖性和频率依赖性．结果提示Ｐｒａ－Ａ对钙电流的阻断作用可能是白花前胡抗心律失常作用的机理之一；Ｐｒａ－Ａ可能对快速型心律失常有防治作用．?-Praeruptorin A (Pra-A) is an effective component of Peucedanum praeruptorum Dunn, a Chinese traditional medicine. In present study, the effects of Pra-A on calcium current (I Ca ) in single ventricular cells of guinea pig heart were examined by using whole-cell patch clamp technique. The results showed that Pra-A (1, 10 and 100 μmol·L -1 ) decreased I Ca in a concentration-dependent manner, and that Pra-A (10 μmol·L -1 ) inhibited I Ca in a use-dependent and a frequency-dependent manner. These results suggested that Pra-A had a calcium channel blocking effect and Pra-A might be promising in the treatment of tachyarrhythmia.

关 键 词：白花前胡素 中药药理 心肌细胞 钙电流 豚鼠 

分 类 号：R285.5[医药卫生—中药学] R282.710.5[医药卫生—中医学]