用牛免疫缺陷病毒筛选抗艾滋病药物的实验模型  

An experimental model for screening anti-AIDS drugs with bovine immunodeficiency virus

作　　者：王岱[1,2] 耿运琪[1,2] 徐为人[1,2] 魏月芳[1,2] 

机构地区：[1]南开大学生命科学学院 [2]国家医药管理局天津药物研究院

出　　处：《中国药理学与毒理学杂志》1996年第1期49-52,共4页Chinese Journal of Pharmacology and Toxicology

摘　　要：本文应用牛免疫缺陷病毒（ＢＩＶ）合胞体形成和ＢＩＶ长末端重复序列（ＬＴＲ）引导的萤火虫荧光素酶（Ｌｕｃ）基因表达分析，建立了抗艾滋病（ＡＩＤＳ）药物的筛选和评价模型．ＢＩＶ合胞体的形成及ＢＩＶ－ＬＴＲ引导的Ｌｕｃ基因活力与被ＢＩＶ感染的细胞数呈线性关系．临床有效药物３′－叠氮胸腺嘧啶脱氧核苷（ＡＺＴ）可显著抑制ＢＩＶ合胞体的形成及ＢＩＶ－ＬＴＲ引导的Ｌｕｃ基因表达水平，并有剂量依赖关系．上述两种分析得到ＡＺＴ的ＩＣ５０分别为０２４和００５２μｍｏｌ·Ｌ－１．The bovine immunodeficiency virus (BIV) induced syncytium formation assay and BIV long terminal repeat (LTR) directed luciferase (Luc) gene expression assay were applied to screen and evaluate anti-AIDS drugs. Frequency of the syncytium formation and BIV-LTR directed Luc activity were in proportion to the number of input BIV-infected cells. AZT inhibited the syncytium formation and the BIV-LTR directed Luc gene expression level. The inhibitory effect was dose-dependent with IC 50 0 24 and 0 052 μmol·L -1 respectively.

关 键 词：艾滋病 抗艾滋病药物 药物筛选 牛免疫缺陷病毒 

分 类 号：R965.1[医药卫生—药理学] R978.7[医药卫生—药学]