10－羟基－2－癸烯酸对小鼠T淋巴细胞及其亚型和白介素2产生的影响  

Effects of 10-hydroxy-2-decenoic acid on T lymphocyte and its subtypes and interleukin 2 production in mice

作　　者：王国燕[1,2] 林志彬[1,2] 

机构地区：[1]北京医科大学基础医学院药理学系 [2]昆明医学院药理学教研室

出　　处：《中国药理学与毒理学杂志》1996年第1期53-55,共3页Chinese Journal of Pharmacology and Toxicology

摘　　要：观察了１０－羟基－２－癸烯酸（１０－ＨＤＡ）对小鼠Ｔ淋巴细胞及其亚型和白介素２产生的影响．结果表明，１０－ＨＤＡ１，５，２５ｍｇ·ｋｇ－１·ｄ－１ｉｐ，５ｄ可拮抗环磷酰胺１００ｍｇ·ｋｇ－１对小鼠迟发型超敏反应（ＤＴＨ）的抑制作用．体外给药，１０－ＨＤＡ可促进刀豆球蛋白Ａ诱导的Ｔ淋巴细胞增殖反应；促进小鼠脾细胞产生白介素２．采用单克隆抗体间接免疫荧光法证明１０－ＨＤＡ可增加小鼠胸腺Ｌ３Ｔ＋４细胞数，而对Ｌｙｔ－２＋细胞无明显影响．结果提示１０－ＨＤＡ可调节Ｔ淋巴细胞参与的免疫反应．Hydroxy-2-decenoic acid (10-HDA), an unsaturated fatty acid, was extracted from royal jelly. The effects of 10-HDA was studied on T lymphocyte functions in mice. The results showed that 10-HDA (1, 5, 25 mg·kg -1 , ip×5 d) significantly antagonized the inhibition of delayed type hypersensitivity (DTH) induced by cyclophosphamide, but it had no effect on DTH in normal mice. The mitogen concanavalin A induced proliferation of T lymphocyte from the mouse spleen was increased by 50 and 100 mg·L -1 10-HDA in vitro. The effect of 10-HDA on the T subpopulation was studied by monoclonal antibody indirect immunofluorescence method. The results showed that 10-HDA (50 and 100 mg·L -1 ) enhanced the number of L 3T + 4 (T H) lymphocytes. 10-HDA (25, 50 and 100 mg·L -1 ) with concanavalin A increased the IL-2 secretion from mouse splenocytes in a concentration-dependent manner. These results suggested that 10-HDA had an immunomodulatory effect on T lymphocyte functions.

关 键 词：蜂王浆 癸烯酸 药理作用 T淋巴细胞 白细胞介素 

分 类 号：R977.9[医药卫生—药品] R967[医药卫生—药学]