L-半胱氨酸衍生物配体交换手性色谱固定相  

A L-Cysteine Derivative as Ligand-exchange Chiral Stationary Phase for Liquid Chromatography

作　　者：王圣庆[1] 孟庆华[1] 郭瑛[1] 马言顺[1] 龙远德[1] 黄天宝[1] 

机构地区：[1]中国科学院成都有机化学研究所

出　　处：《高等学校化学学报》2005年第9期1631-1633,共3页Chemical Journal of Chinese Universities

摘　　要：A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl octyl ether and then coating it on YWG-C-{18} bonded stationary phase. The enantiomeric resolutions of some D,L-α-amino acids were achieved on CysCSP by using cupric acetate aqueous solution as the mobile phase, under the conditions of column temperature 20 ℃ and detection at UV 254 nm. The enantioselectivities α of D,L-α-amino acids separated were found to be in the range from 1.11 to 1.38 with the resolution R-s ranging from 1.1 to 2.8 and the column efficiency being from 5 000 to 9 000 n/m. The elution order of D-isomer before L-isomer was obersaved for all D,L-amino acids used.A novel chiral stationary phase （CysCSP） for ligand exchange chromatography was prepared by firstly synthesizing N,S-di（2-hydroxyl-3-octoxyl）propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl octyl ether and then coating it on YWG-C18 bonded stationary phase. The enantiomeric resolutions of some D,L-α-amino acids were achieved on CysCSP by using cupric acetate aqueous solution as the mobile phase, under the conditions of column temperature 20 ℃ and detection at UV 254 nm. The enantioselectivities at of D, L-α-amino acids separated were found to be in the range from 1.11 to 1.38 with the resolution Rs ranging from 1.1 to 2.8 and the column efficiency being from 5 000 to 9 000 n/m. The elution order of D-isomer before L-isomer was obersaved for all D,L-amino acids used.

关 键 词：配体交换色谱 手性拆分 L-半胱氨酸 D L-α-氨基酸 

分 类 号：O657[理学—分析化学]