上海市居室尘螨变应原浓度初步检测  

Preliminary Survey on Concentration of Indoor Allergens Derived from Dust Mites in Residential Homes in Shanghai

作　　者：毛佐华[1] 刘施巍[1] 张凤霞[1] 周淑君[1] 何玉良[1] 

机构地区：[1]复旦大学医学院寄生虫学教研室,上海200032

出　　处：《中国寄生虫学与寄生虫病杂志》2005年第4期225-227,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的检测上海市居室尘螨变应原浓度.方法 应用层析介质及蛋白质沉淀技术从螨代谢分泌物中分离、纯化粉尘螨变应原Dff F3及国际公认的变应原Der fⅢ,经临床检测和放射性变应原吸附实验(RAST)鉴定其变应原性.制备抗Dff F3和Der fⅢ IgG,从室内收集尘埃,分离尘螨抗原,夹心ELISA测定尘螨变应原Dff F3和Der fⅢ浓度.结果 Dff F3和Der fⅢ呈现强致变应性,均能与尘螨过敏哮喘患者血清IgE结合.检测200份家庭尘埃样品尘螨变应原,结果显示,Dff F3浓度>10 μg/g的家庭占57.0%,2～10 μg/g的家庭占29.5%,<2 μg/g的家庭占13.5%.Der fⅢ浓度>10 μg/g的家庭占53.5%,2～10 μg/g的家庭占32.0%,<2 μg/g的家庭占14.5%.结论 上海市部分居室尘螨变应原浓度偏高.粉尘螨Dff F3有较强的变应原性.Objective To investigate the level of indoor dust mite allergens in residential homes in Shanghai. Methods The allergenic proteins, Dff F3 and Der f Ⅲ （well known allergen） from the whole culture extract of Dermatophagoides farinae were purified by chromatography and sedimentation techniques. Allergic activities of both proteins were identified by skin prick test and RAST. After preparation of specific IgG anti-Dff F3, anti-Der f Ⅲ , and extraction of indoor allergens from 200 indoor dust samples collected from residential homes, the allergen concentration was measured by sandwich ELISA. Allergen level was expressed in geometric mean and range, the analysis of variance （ANOV） was used to determine the level of significance between groups. Results Dff F3 and Der f Ⅲ were demonstrated strongly allergic activities, which can be highly recognized with IgE from sera of the mite-allergic patients with asthma. In the sampled 200 homes, the proportion of homes with Dff F3 level of 〉10 μg/g was 57.0%, 29.5% for group of 2—10 μg/g, and 13.5% for group of 〈2 μg/g. The proportion of homes with Der f Ⅲ level of 〉10 μg/g was 53.5%, 32.0% for group of 2—10 μg/g, 14.5% for group of 〈2 μg/g. Conclusion Selected residential homes in Shanghai were found more likely to have high level of dust mite allergens. Dff F3 was identified as the stronger allergic fraction.

关 键 词：粉尘螨 变成原 居室 

分 类 号：R757.3[医药卫生—皮肤病学与性病学]