自身启动子调控下小麦Anti-gbssⅠ基因表达载体的构建及转基因植株的培养  

Construction of Wheat Anti_gbssⅠExpression Vector with Its Promoter and Cultivation of Transgenic Plants(Triticum aestivum L.)

作　　者：苗红梅[1] 化青报[2] 李利红[3] 赵永英[1] 易明林[1] 郅玉宝[1] 

机构地区：[1]河南省农业科学院生物技术研究所,河南郑州450002 [2]河南农业大学,河南郑州450002 [3]郑州牧业工程高等专科学校生物工程系,河南郑州450011

出　　处：《河南农业大学学报》2005年第3期243-248,共6页Journal of Henan Agricultural University

基　　金：河南省杰出人才创新基金项目(0121000700).

摘　　要：利用反义技术,构建了含有gbssⅠ启动子和自身反义基因序列的表达载体pRgbss,通过基因枪法在小麦中转化和PCR检测,共得到阳性转基因植株3株.The pRgbss expression vector containing gbss Ⅰ promoter and its antisense gene sequence was constructed using Antisense technique, and transformed into wheat scutella with particle bombardment technique. Three independent transgenic plants were obtained and assayed using PCR for further researchs on wheat starch quality improvement.

关 键 词：小麦 淀粉 gbssⅠ启动子 gbssⅠ反义基因 转化 

分 类 号：S512.1[农业科学—作物学]