高效液相色谱法同时测定复方葡萄糖酸钙口服溶液中2组分的含量  

Simultaneous Determination of2Components in Compound Calcium Gluconate Oral Solution by HPLC

作　　者：孙祥德[1] 高革[1] 赵俊环[2] 齐伟[1] 刘建超[3] 

机构地区：[1]河南新乡医学院分析测试中心,新乡市453003 [2]河南新乡医学院附属第三医院,新乡市453003 [3]河南三门峡职业技术学院,三门峡市472000

出　　处：《中国药房》2005年第17期1334-1335,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱法同时测定复方葡萄糖酸钙口服溶液中乳酸钙和葡萄糖酸钙含量的方法。方法:色谱柱为C18,流动相为0.025mol/LNaH2PO4(pH=2.50),检测波长为210nm,流速为1.0ml/min,柱温为25℃,进样量为20μl。结果:乳酸、葡萄糖酸检测浓度分别在0.719～46.040(r=0.9999)、0.315～20.160(r=0.9999)mmol/L范围内线性关系良好;平均加样回收率分别为99.0%(RSD=1.65%)、100.5%(RSD=1.36%)。结论:本方法快速、简便、准确,且可不经分离直接同时测定本品中乳酸钙和葡萄糖酸钙的含量,可用于复方葡萄糖酸钙口服溶液的质量控制。OBJECTIVE： To establish HPLC method for the simultaneous determination of calcium lactate and calcium gluconate in compound calcium gluconate oral solution.METHODS： The test was performed on Cla column with column temperature at 25℃ ;the mobile phase was composed of 0.025mol/L NaH2PO4（pH = 2.50） with a flow rate of 1.0ml/min and sample size of 20μl;the detection wavelength was 210nm. RESULTS：The calibration curves were linear over the range of 0. 719～ 46.040rnmol/L（r = 0.9 999） for lactic acid and 0.315～20.160mmol/L（ r = 0.9 999） for gluconic acid .The average recovery rates of which were 99.0 % （RSD = 1.65 % ） and 100.5 % （RSD = 1.36% ）, respectively. CONCLUSION ： This method is fast, simple, accurate, and it can be employed directly without isolation to determine the content of calcium lactate and calcium gluconate simultaneously and to control the quality of compound calcium gluconate oral solution as well.

关 键 词：高效液相色谱法 乳酸钙 葡萄糖酸钙 含量测定 

分 类 号：R927.2[医药卫生—药学]