高效液相色谱法测定参芍肝康口服液中芍药苷的含量  

Determination of Peoniflorin in Shenshaogankang Oral Liquid by HPLC

作　　者：李宏亮[1] 吴金虎[1] 肖雨清[1] 雷龙[1] 

机构地区：[1]湖北十堰市人民医院药学部,十堰市442000

出　　处：《中国药房》2005年第17期1336-1337,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱法测定参芍肝康口服液中芍药苷含量的方法。方法:色谱柱为KromasilC18,流动相为甲醇-0.05mol/L磷酸二氢钾溶液-异丙醇(60∶173∶4,用醋酸调节pH值至4.0),检测波长为230nm,流速为1.0ml/min。结果:芍药苷检测浓度在0.027～0.230mg/ml范围内与峰面积积分值线性关系良好(r=0.9999);平均加样回收率为100.97%(RSD=1.28%,n=5)。结论:本方法简单、快速、精密度高、重现性好,可用于参芍肝康口服液的质量控制。OBJECTIVE： To establish HPLC method for the determination of the content of paeoniflorin in Shenshaogankang oral liquid. METHODS： Paeoniflorin was separated on Kromasil C18; methanol -0.05mol/L KH2PO4 solution- avantin （60 ： 173 ： 4,which was adjusted to pH = 4.0 with acetic acid）was taken as the mobile phase with a flow rate at 1.0ml/min and detection wavelength at 230nm.RESULTS： Good linear relationship was achieved when the detection concentration range of peoniflorin was 0.027～0.230mg/ml（ r = 0.9 999） ; The average recovery of peoniflorin was 100.97% （RSD = 1.28%, n = 5）. CONCLUSION ：The method was simple, rapid,precise and reproducible,which can be used as the quality control of Shenshaogankang oral liquid.

关 键 词：高效液相色谱法 参芍肝康口服液 芍药苷 含量 

分 类 号：R927.2[医药卫生—药学]