多重PCR筛选临床细菌耐药整合子基因  

Multi-PCR assay to screen antibiotic resistance integron genes in clinical isolates of bacteria

作　　者：肖增璜[1] 石磊[2] 邱春嫦[1] 付强[1] 苏健裕[2] 冯洁[2] 陈洵[2] 李琳[2] 

机构地区：[1]暨南大学附属第一医院临床检验中心,广东广州530630 [2]华南理工大学轻工与食品学院,广东广州510640

出　　处：《暨南大学学报（自然科学与医学版）》2005年第4期480-485,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省自然科学基金项目(04020050);广州市科技计划项目(2004J1-C0161)

摘　　要：目的:建立多重PCR方法并对临床菌株进行扩增及整合子分类。方法:采用多重PCR对21株来自临床的耐药菌株进行了第一、二和三类整合酶基因(intI)筛选。结果:16株携带第一类整合酶基因;1株携带第一和第二类整合酶基因;2株含第二类整合酶基因;1株含第三类整合酶基因;1株不含第一、二和三类整合酶基因。结论:筛选细菌耐药整合子基因分类的多重PCR方法简便可靠;整合子广泛存在于临床耐药细菌中。Aim： To construct a multi - PCR assay using specific primers of intl, int2 and int3 to screen class 1,2 and 3 integrase （intⅠ） among clinical samples. Methods： of 21 strains isolated from clinical source were detected by multi - PCR in this study. Results： Among 21 strains, 16 strains were shown harbored /ntl. Of 1 strain was found to carry intl and int2 genes. Of 2 strains were positive for int2 gene. Of 1 strain was positive for int3 gene. Of 1 strain was negative in multi - PCR. Conclusion： A multi - PCR assay to screen class 1,2 and 3 integron at one time was constructed and confirmed to be practicable. The result shows that integrons have widely existed in antibiotic resistance clinical isolates of bacteria.

关 键 词：多重PCR 整合子 整合酶基因 耐药菌株 酶基因 合子 临床 细菌 筛选 PCR方法 

分 类 号：Q75[生物学—分子生物学] S948[农业科学—水产养殖]