选择性COX-2抑制剂尼美舒利对白血病K562细胞增殖的影响  

Effects of nimesulide on proliferation in leukemia K562 cells

作　　者：吕艳青[1] 叶春玲[2] 叶开和[2] 刘建军[3] 任先达[1] 

机构地区：[1]暨南大学药学院临床药理学教研室,广东广州510632 [2]暨南大学药学院药理学教研室,广东广州510632 [3]暨南大学药学院实验技术中心,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2005年第4期502-507,共6页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：目的:观察选择性COX-2抑制剂尼美舒利对人类骨髓白血病K562细胞增殖和凋亡的影响。方法:分别以尼美舒利25、50、100μg.mL-1体外处理骨髓白血病K562细胞,采用MTT比色法、流式细胞仪分析技术及DNA片段凝胶电泳等方法,检测尼美舒利对K562细胞增殖和凋亡的影响。结果:尼美舒利剂量依赖性地抑制K562细胞增殖,其中等剂量组(50μg.mL-1)的抑制作用强于阿霉素组(25μg.mL-1);尼美舒利可诱导细胞凋亡,使细胞阻滞于G0/G1期,S期细胞明显减少,其诱导细胞凋亡的作用也呈剂量依赖性(剂量由低至高,凋亡率分别为(3.4±2.8)%,(16.8±1.8)%和(42.7±2.5)%;DNA条带分析也进一步证实了尼美舒利的促凋亡作用。结论:尼美舒利在体外可显著抑制K562细胞增殖,该作用可能与其干扰细胞周期、诱导细胞凋亡有关。Aim： To explore the effects of nimesulide, a selective COX - 2 inhibitor, on the proliferation and apoptosis in leukemia K562 cells. Methods： Cell viability, cell cycle and the apoptosis of K562 were detemfined by MTT assay, flow cytometry and DNA ladder analysis. K562 cells were separately treated with different dosages of nimesulide （25, 50, 100 μg·mL^-1） in vitro. Results： The cell proliferation was inhibited significantly by nimesulide, in a dosedependent mariner. At the middle dosage group （50μg·mL^-1）, the inhibitive effect of nimesulide was greater than that of adriamycin （25μg· mL^-1）. After exposure to different concentrations of nimesulide for 48 h, nimesulide elicited proapoptotic effects, and K562 ceils were accumulated in C0/G1 phase, decreased in the number of cells in S phase. Its proapoptotic effect was also in a dose - dependent manner （from low to high dosage, the apoptotic rates were 3.4 ± 2.8, 16.8 ± 1.8 and 42.7 ± 2.5 percent, respectively）. The DNA ladder analysis further verified the proapototie effects of nimesulide. Conclusion： Nimesulide significantly inhibits the proliferation of K562 cell in vitro, and it may exert this proliferation - inhibiting action via inducing apoptosis and blocking cell cycle progression.

关 键 词：非甾体抗炎药 尼美舒利 阿霉素 白血病 凋亡 

分 类 号：R96[医药卫生—药理学]