自发性流产CBA/J×DBA/2小鼠母-胎界面DX5^+CD69^+细胞的浸润  

Infiltration of DX5^+CD69^+ cells at the feto-maternal interface in spontaneous abortion CBA/J×DBA/2 mice

作　　者：林羿[1] 曾耀英[1] 狄静芳[1] 曾山[1] 曾祥凤[1] 詹美意[2] 

机构地区：[1]暨南大学组织移植与免疫研究中心,广东广州510632 [2]暨南大学医学院病理学教研室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2005年第4期508-512,529,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：中国博士后科学基金(2002032244);暨南大学引进优秀人才科研启动基金(51204067);广东省自然科学基金(4300213)资助项目

摘　　要：目的:研究DX5+CD69+细胞浸润与CBA/J×DBA/2小鼠自发性胚胎吸收的关系。方法:CBA/J×DBA/2小鼠(A组)和CBA/J×BALB/c小鼠(B组)分别用作自发性流产模型和生育力正常对照。DX5用作广泛性NK细胞标志物,CD69作为NK细胞和T细胞活化标志物。采用流式细胞术检测母-胎界面淋巴细胞中CD69+细胞百分率,DX5+CD69+细胞与DX5+细胞比率,并对细胞数量与胚胎吸收率进行线性相关回归分析。结果:A组孕13.5 d胚胎吸收率为34.1%(45/132),显著高于B组(6.6%,9/137;P<0.001)。同时A组DX5+CD69+细胞与DX5+细胞比率(28.1±11.9)%显著高于B组(19.5±5.7)%,(P<0.01)。此外,A组DX5+CD69+与DX5+细胞比率(x)和胚胎吸收率(y)之间存在强线性相关(r=0.807,P<0.001,y=-1.157+1.278x)。结论:母-胎界面过多的DX5+CD69+细胞浸润可能与CBA/J×DBA/2小鼠胚胎吸收有关。Aim： To investigate whether DX5^＋ CD69^＋ cells are involved in recurrent spontaneous resorption of embryos in CBA/J female mouse mated with DBA/2 male （CBA/J × DBA/2）. Methods： CBA/J × DBA/2 （group A） and CBA/J × BALB/c （group B） were used as abortion - prone model and fertile control, respectively. DX5 was used as a pan - NK cell marker, while CD69 was used as an activation marker on NK and T cells. The proportion of CD69^＋ cells in lymphocyte group, and that of DX5^＋ CD69^＋/DX56＋ at the feto- matemal interface were determined with flow cytometry. Linear correlation analysis was also performed between the cell number and resorption rate （RR）. Results： RR was 34.1% （45 out of 132） in group A on day 13.5 of gestation. Such level was significantly higher than group B （6.6% ; 9 out of 137; P 〈 0.001）. Similarly, the ratio of DX5^＋ CD69^＋/ DX5^＋ cell was also significantly higher in group A （28.1 ± 11.9） % than in group B （ 19.5 ± 5.7） %, （ P 〈 0.01）. In addition, a strong linear correlation was observed between DX5^＋ CD69 ^＋/DX5 ^＋ cell ratio （x） and RR （y） （r =0.807, P 〈0.001, y = -1.157 ＋ 1.278x）in group A. Conclusion： Infiltration of excessive DX5 ^＋ CD69 ^＋ cells at the feto - matemal interface may be associated with recurrent embryo resorption in CBA/J × DBA/2 mice.

关 键 词：流产 动物模型 细胞表面标志物 淋巴细胞 

分 类 号：R392.32[医药卫生—免疫学]