二种中药复方对慢性心理应激大鼠行为及下丘脑-垂体-肾上腺轴的影响  

The effect of two kinds of traditional Chinese herbal compound on the behavior and hypothalamus-pituitary-adrenal gland axis of the chronic psychological stress rats

作　　者：邹军[1] 梁尚华[1] 赵仓焕[2] 倪力强[3] 曹碧茵[1] 陈慧娟[1] 童瑶[4] 

机构地区：[1]上海中医药大学基础医学院,上海201203 [2]暨南大学附属第一医院中医科,广东广州510630 [3]上海市食品药品监督管理局科技情报研究所,上海200040 [4]香港大学中医药学院

出　　处：《暨南大学学报（自然科学与医学版）》2005年第4期530-534,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：上海市科学技术委员会基础研究重点项目(02JC14024)

摘　　要：目的:观察半夏厚朴汤、桂枝甘草龙骨牡蛎汤对慢性心理应激大鼠行为及下丘脑-垂体-肾上腺轴的影响。方法:将SD大鼠分为5组:正常组、模型Ⅰ组(慢性心理应激模型,不灌服生理盐水)、模型Ⅱ组(慢性心理应激模型,灌服生理盐水)、半夏厚朴汤组(灌服半夏厚朴汤水煎浓缩液)、桂枝甘草龙骨牡蛎汤组(灌服桂枝甘草龙骨牡蛎汤水煎浓缩液),连续灌服19 d测试各组大鼠行为、下丘脑促肾上腺皮质激素释放激素(CRH)、血浆促肾上腺皮质激素(ACTH)及血清皮质酮(CORT)。结果:半夏厚朴汤组大鼠第11、19 d爬格次数增加,第11 d挣扎次数增加,CRH、ACTH降低;桂枝甘草龙骨牡蛎汤组大鼠第19 d爬格次数增加,第19 d静止时间缩短,CRH、ACTH降低。结论:半夏厚朴汤、桂枝甘草龙骨牡蛎汤可作用于下丘脑-垂体-肾上腺轴的不同环节,一定程度上影响慢性应激大鼠的行为方式,调节慢性应激大鼠的内分泌功能。Aim： To study the effects of Banxia Houpu Soup and Guizhi Gancao Longgu Mull Soup on the behaviors and HPA axis of chronic psychological stress rats. Methods： SDrats were divided into 5 groups： normal group, model groups Ⅰ and Ⅱ, Banxia Houpu Soup group and Guizhi Gancao Longgu Muli Soup group. The behavior, the hypothalamus CRH, plasma ACTH and serum CORT（radio - immunity method） of all the rats were observed. Resuits： On the 11 th day, the crossing times of model group Ⅰ and the crossing times and struggle time of model group Ⅱ changed obviously compared with those of normal group. On the 19th day, the crossing times and still time of model group Ⅰ and Ⅱ had remarkable differences, while the contents of CRH, ACTH of model group Ⅰ and the contents of CRH, ACTH and CORT of model group Ⅱ increased obviously compared with those of normal group. Compared with those of model group Ⅰ and Ⅱ, the crossing times on the llth andl9th days and the struggle times on the 11th day of Banxia Houpu Soup group increased obviously, while the contents of CRH and ACTH decreased; the crossing times on the 19th day increased, but the still time on the 19th day shortened and thecontents of ACTH and CRH decreased in Guizhi Longgu Muli Soup. Conclusion： Banxia Houpu Soup and Guizhi Longgu Muli Soup can affect the behavior and the endocrinal system of the chronic psychological stress rats in a certain level by through the action on the different sectors of the hypothalamus - pituitary - adrenal gland axis.

关 键 词：慢性心理应激 旷场试验 尾悬挂试验 下丘脑-垂体-肾上腺轴 半夏厚朴汤 桂枝甘草龙骨牡蛎汤 

分 类 号：R2-03[医药卫生—中医学]