天津地区汉坦病毒分离株TJJ16 S基因片段克隆和序列分析  

Cloning and sequencing of S segment of hantavirus strain TJJ16 isolated in Tianjin

作　　者：李力[1] 杨东靖[1] 陈锦英[2] 丁建清[1] 苏旭[1] 田永琴[1] 魏骏飞[2] 

机构地区：[1]天津市卫生防病中心,天津医科大学300011 [2]天津医科大学

出　　处：《中华微生物学和免疫学杂志》2005年第8期652-655,共4页Chinese Journal of Microbiology and Immunology

基　　金：天津市科技发展计划项目(043113511)

摘　　要：目的了解天津地区汉坦病毒分离株TJJ16S基因的分子特征。方法采用细胞培养法从鼠肺标本中分离汉坦病毒,命名为TJJ16,提取TJJ16感染VeroE6细胞的总RNA,经逆转录扩增病毒cDNA,继而进行PCR扩增S基因片段,纯化后克隆于pMD18T载体,测定核苷酸序列并进行分析。结果天津地区首次从社鼠中分离出汉坦病毒TJJ16,其S基因片段全序列长为1701nt。只有1个编码N蛋白的开放读码框架(ORF),起始密码子为第37nt,终止于1326nt,推导其编码的蛋白长为429个氨基酸。经核苷酸系统发生树分析,TJJ16株应归属为汉坦病毒的汉滩型(HTN型)毒株,与HTN型新亚型A16株同属一个亚型。结论TJJ16病毒株的分离与S基因片段序列分析,证实在天津市鼠间存在HTN型汉坦病毒感染,对临床和流行病学的研究具有重要意义。Objective To study the molecular character of S gene segment from hantavirus strain TJJ16 isolated in Tianjin. Methods The hantavirus strain was isolated and total cellular RNA were extracted from Vero-E6 cells infected with hantavirus. Viral S gene segment was amplified by reverse transcripase polymerase chain reaction （RT-PCR） and cloned into pMD-18T vector. The S segment was sequenced. Results It is the first isolation of hantavirus from host-Rattus co.Janus in Tianjin. The S segment was 1701 nt in length; only one ORF （from 37 nt to 1326 nt） encoded 429 amino acids. Phylogenetie tree showed that TJJ16 strain belongs to HTN type vires, the same subtype as A16 strain. Conclusion HTNV infection was proved by the isolation and sequence of TJJ16 vires strain in Tianjin. It is very important for study on clinical research and epidemiology.

关 键 词：汉坦病毒 S基因片段 序列分析 病毒分离株 天津地区 汉坦病毒感染 VERO-E6细胞 核苷酸序列 克隆 开放读码框架 

分 类 号：R373[医药卫生—病原生物学]