护肾固精方预防大鼠同种慢性移植物肾病的研究  

Efficacy of Hushen Gujing (HSGJ) in preventing chronicallograft nephropathy in rats

作　　者：田晓辉[1] 薛武军[1] 王志勇[2] 周清发[2] 张寅生[1] 丁小明[1] 田普训[1] 

机构地区：[1]西安交通大学第一医院,陕西西安710061 [2]西安交通大学第二医院,陕西西安710004

出　　处：《中国中药杂志》2005年第17期1349-1352,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30271623)

摘　　要：目的:探讨护肾固精方对慢性移植物肾病的影响。方法:以Fisher大鼠作供者,Lewis大鼠作受者进行同种肾移植。移植后治疗组大鼠隔日给予护肾固精方饲喂。移植后16周作肾功能、移植肾组织学检测,测定肾组织中转化生长因子mRNA的表达。结果:与对照组比较,移植后治疗组24 h尿蛋白持续保持较低的水平(P<0.01),血清肌酐水平较低(P<0.01),肌酐清除率高(P<0.01);移植肾肾小球硬化程度轻(P<0.01),Banff评分低(P<0.01);转化生长因子β1(TGF-β1)的mRNA表达减少(P<0.01)。结论:护肾固精方对移植肾功能有保护作用,其机制与影响TGFβ1在移植肾的表达有密切关系。Objective： To evaluate the effect of HSGJ on chronic allografi nephropathy （CAN） using standaxd rat model of CAN. Method： Renal transplantation was performed with Fisher rats as donors and Lewis rats as recipients. All the recipients were randomly divided into control group and medication groups （high and low dosage of HSGJ, fed every other day）. After 16 weeks of treatment, renal function and the histological alteration of CAN were measured. The expression of the TGβ1 mRNA in the aUograft was evaluated by real-time PCR. Result： The content of 24 h urine protein and the level of serum creatinine in the medication groups were significantly decreased （P〈0.01） as compared with control group, whereas the creatinine clearance was increased（P〈0.01）. The degree of glomerular sclerosis and the Banff score of medication groups were lower than the control group respectively （P〈0.01）, in consistent with decreased expression of the TGF lmRNA. Conclusion： HSGJ can prevent the chronic allograft nephropathy and the mechanism may be related with its influence on the expression of the TGFβ1.

关 键 词：肾移植 慢性移植物肾病 护肾固精方 转化生长因子Β1 

分 类 号：R285[医药卫生—中药学]