白首乌多糖-1a的免疫调节作用研究  

Studies on immunoregulation of polysaccharides-1a from Radix Cynanchi Bungei

作　　者：高丽君[1] 王建华[2] 崔建华[3] 王汉忠[2] 

机构地区：[1]泰山医学院药学院,山东泰安271000 [2]山东农业大学农学院,山东泰安271018 [3]泰山医学院化学与化学工程学院,山东泰安271000

出　　处：《中国中药杂志》2005年第17期1352-1355,共4页China Journal of Chinese Materia Medica

基　　金：山东省优秀中青年科学家科研奖励基金资助项目(03BS049)

摘　　要：目的:研究白首乌多糖(PRCB)组分PRCB1a体外对兔T淋巴细胞转化及体内对正常小鼠免疫功能的影响。方法:将PRCB1a分为2,4,6 g.L-13个剂量组,分别加入含有兔血的营养瓶内,PHA体外诱导,通过观察T淋巴细胞的转化情况,研究该多糖体外免疫功能状况;将PRCB1a分为3个剂量(150,100,50 mg.kg-1.d-1),连续给小鼠ig 7 d,并进行小鼠体内免疫功能调节作用的测试。结果:PRCB1a各剂量组,均能极显著地(P<0.01)促进PHA诱导的家兔T淋巴细胞在体外的增殖能力;PRCB1a并能显著增强小鼠的胸腺和脾脏指数、巨噬细胞的吞噬功能、增强小鼠迟发型超敏反应、加速碳粒的清除。结论:提示白首乌多糖能提高小鼠的非特异性和特异性细胞免疫功能。Objective： To study the effects of PRCB1 a（one component of polysaccharides from Radix Cynanchi Bungei）on transformation of T lymphocytes of rabbit in vitro and immune function in mice. Method： Three doses of PRCBla （2,4,6g·L^-1） were respectively put in bottle with PHA and blood of rabbit. The effect of PRCBla on immunity in vitro was studied by observing transformation of T lymphocytes; The dosage of PRCB1a （50,100,150 mg·kg^-1·d^-1） was given orally for seven days. The effects on immune function were investigated in mice. Result： Three doses of PRCBla could significantly promote （P〈0.01） the ability of T lymphocytes proliferation; PRCBla could improve the mouse thymus and spleen index, the celiac macrophage ability of engulfing CRBC, the delayed type hypersensitivity ability and the macrophage engulfing carbon granula ability. Conclusion： The results indicate PRCBla can enhance nonspectific and specific cellular immune function.

关 键 词：白首乌 多糖 免疫调节 迟发型超敏反应 碳廓清试验 

分 类 号：R285[医药卫生—中药学]