大豆疫霉菌抗甲霜灵特性研究  

STUDIES ON RESISTANCE OF PHYTOPHTHORA SOJAE TO METALAXYL

作　　者：左豫虎[1] 侯巨梅[2] 康振生[3] 陈长卿[3] 黄丽丽[3] 

机构地区：[1]西北农林科技大学植保学院,陕西杨凌712100 [2]黑龙江八一农垦大学植物科技学院,大庆163319 [3]西北农林科技大学植保学院陕,西杨凌712100

出　　处：《菌物学报》2005年第3期422-428,共7页Mycosystema

基　　金：国家重点基础研究发展规划(2002CB111406);国家杰出青年基金项目(30125031);西北农林科技大学"创新团队"项目

摘　　要：大豆疫霉Phytophthorasojae易对甲霜灵产生抗性,从大豆疫霉野生型菌株可诱变筛选到对甲霜灵有抗性的菌株Mtr。Mtr抗性菌株的抗性水平可达野生型单游动孢子菌株的870倍以上。Mtr性状在无性后代稳定遗传,在游动孢子后代连续三代未发生抗药性分离。大豆疫霉Mtr性状的保持对甲霜灵没有表现依赖性。Mtr单游动孢子菌株在不含甲霜灵的胡萝卜培养基(CA)平板上培养30d后对甲霜灵的抗性没有下降,其单游动孢子后代也未出现抗药性分离。Phytophthora sojae developed the resistance to metalaxyl easily. The metalaxyl-resistant isolate （Mt^s） of P. sojae was found in the wild-type isolates of P. sojae after treatment with metalaxyl. The resistance level of the metalaxyl-resistant isolate was over 870 times higher than that of the single zoospore progeny isolates of the wild-type parent isolate. The resistance to metalaxyl of Mt^s isolate was inherited stably, and they did not show variation from the first zoospore progeny to the third zoospore progeny. The maintenance character of Mt^s was not depended on metalaxyl. The resistance to metalaxyl of Mt^s single zoospore isolate was not reduced and their zoospore progeny were not varied after 22-30 days growth on the metalaxyl free carrot agar medium （CA）.

关 键 词：抗甲霜灵菌株 抗药性 遗传 

分 类 号：S481.4[农业科学—农药学]