浙江无核柿炭疽病菌鉴定及附着胞形成过程中的核相变化  

ANTHRACNOSE PATHOGEN ON DIOSPYROS KAKI CV. WUHESHI AND ITS NUCLEAR BEHAVIOR IN PROCESS OF APPRESSORIUM FORMATION Z

作　　者：张敬泽[1] 徐同[1] 何黎平[2] 

机构地区：[1]浙江大学生物技术研究所 [2]浙江大学分析测试中心,杭州310029

出　　处：《菌物学报》2005年第3期446-456,共11页Mycosystema

基　　金：杭州市科委资助(杭科计发字2000122G59)

摘　　要：浙江无核柿炭疽病近年来在浙江淳安地区严重发生,根据形态学特征病原菌鉴定为胶孢炭疽菌Colletotrichum gloeosporioides Penz.,在枝条病斑上的分生孢子盘通常不产生刚毛,分生孢子顶端顶部钝圆,基部平截,分生孢子盘中的孢子包埋在基质中,紧密结合在一起.分生孢子在自然寄主和人工培养条件下形态特征相似.6个柿树炭疽菌菌株的rDNA ITS序列联配显示,其序列是相同的.用UPGMA方法分析ITS1-ITS2序列构建的炭疽菌系统发育树把6个柿树炭疽菌菌株和其它寄主上的胶孢炭疽菌或其有性型围小丛壳菌菌系分入同一个组,与根据形态学的鉴定结果一致.在附着胞形成过程中,用DAPI荧光染色观察到核相发生两次有丝分裂变化.第一次有丝分裂发生在分生孢子固着聚苯乙烯塑料培养皿3-4 h后,随后,分生孢子中部形成一个隔膜,把它分成两个细胞;6～7 h后,分生孢子发生第二次有丝分裂.分裂后,一个核通过芽管移入附着胞中.Anthracnose pathogen on Diospyros kaki cv. Wuheshi in Zhejiang province was identified as Colletotrichum gloeosporioides based on its morphological characters. No difference was found in morphology both on natural host and culture. The setae of acervulus usually were absent on lesions of shoots. Under scanning electron microscopy, apex of conidia is obtuse and the base truncate; conidia were embedded within and surrounded by mucilage. Phylogenetic tree of Collectotrichum isolates by UPGMA analysis of rDNA ITS1-ITS2 sequence allowed 6 isolates from persimmon to cluster into a group together with C gloeosporioides and its teleomorph Glomerella cingulata from other hosts, corresponding with the morphological identification. During appressorium development, mitosis were twice visualized using DNA specific fluorochrome, DAPI. The primary mitosis took place after 3-4 h when conidia settled in the polystyrene cultures. Subsequently a septum forms in the center of the conidium and divides the conidium into two cells. After 6-7 h, the secondary mitosis occurred, and one nucleus moved into the appressorium.

关 键 词：形态学 核糖体DNA序列 胶孢炭疽病菌 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]