赤点石斑鱼病毒性神经坏死症的组织病理和电镜观察  

Histopathological examination and electron microscopy observation of viral nervous necrosis in red-spotted grouper, Epinephelus akaara

作　　者：林蠡[1] 黄剑南[1] 翁少萍[1] 王云新[2] 刘付永忠[2] 张海发[2] 何建国[1] 

机构地区：[1]中山大学生命科学学院生物防治国家重点实验室,广东广州510275 [2]广东省大亚湾水产试验中心,广东惠州526107

出　　处：《水产学报》2005年第4期519-523,共5页Journal of Fisheries of China

基　　金：科技部海洋"863"计划资助项目(2001AA621010);中国国家留学基金会和德国学术交流中心(DAAD)奖学金资助

摘　　要：用逆转录-聚合酶链式反应(RTPCR)检测患病赤点石斑鱼苗,呈Beta诺达病毒阳性。光学显微镜下观察到病鱼的脑、视网膜、脊髓有空泡。在脑部,空泡主要分布在端脑、间脑和小脑。受感染的细胞明显收缩、致密变化和嗜碱性。包涵体常为圆形,大小不一。透射电镜下,在感染细胞的细胞质可观察到含有病毒粒子的致密体。病毒粒子呈等面体,无外膜,直径为25～28nm,随机分布在细胞质或在致密体内排列成晶格状。致密体大小不一。偶尔观察到较大致密体的外膜已破裂,病毒粒子被释放到细胞质。A specific band of Betanodavirus RNA2 was detected from red-spotted grouper（ Epinephelus akaara） larvae by reversetranscription polymerase chain reaction （ RT-PCR）. Under light microscopy, the brain, retina, and spinal cord of affected fish showed conspicuous vacuolation. In the brains, vacuoles were mainly apparent in the telencephalon, the diencephalon and the cerebellum. Affected cells were pyknosis accompanying marked shrinkage and basophilia. Inclusion bodies were usually round and different in size. Transmission electron microscopy revealed affected cells containing dense bodies filled with numerous viral particles in the cytoplasm. Virions were 25 - 28 nm in diameter, icosahedral and non-enveloped. Virions were distributed randomly in the cytoplasm or arranged in crystalline arrays in the dense bodies. The sizes of dense bodies were different. Occasionally, the unit membrane surrounding the larger bodies had ruptured, allowing the viral particles to escape into the cytoplasm.

关 键 词：赤点石斑鱼 Beta诺达病毒 病毒性神经坏死症 

分 类 号：S941.41[农业科学—水产养殖]