醋酸亮丙瑞林辅助排卵对雌二醇和子宫内膜分泌功能的影响  

EfectsofAsistedOvarianStimulationwithLeuprolideAcetateonAlterationsofPlasmaEstradiolLevelsandNewlySynthesizedEndometrialProteins

作　　者：黄荷凤[1,2] 石一复[1,2] 周馥贞[1,2] 杨树标[1,2] 何柏松[1,2] 

机构地区：[1]浙江医科大学附属妇产科医院 [2]香港大学医学院妇产科学系

出　　处：《中华妇产科杂志》1996年第2期103-106,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的：探讨醋酸亮丙瑞林（ＬＡ）对体外受精－胚胎移植周期的影响。方法：对金黄地鼠给予ＬＡ＋孕马血清促性腺激素（ＰＭＳＧ）辅助排卵（ＬＡ＋ＰＭＳＧ组），测定血浆雌二醇水平和子宫内膜新合成蛋白，并与单纯给予ＰＭＳＧ促排卵（ＰＭＳＧ组）以及行自然周期的动物（对照组）进行比较。结果：ＬＡ＋ＰＭＳＧ组雌二醇水平和子宫内膜新合成蛋白区带与对照组相似。ＰＭＳＧ组雌二醇水平呈先高后低状态，子宫内膜新合成蛋白明显下降。结论：ＬＡ＋ＰＭＳＧ辅助排卵对垂体起降调节作用，使子宫内膜发育趋向生理化；不同的排卵方法对子宫内膜的蛋白合成效应不同。Objective:Toevaluatetheefectsofleuprolideacetate(LA)-asistedovarianstimulationonendometrialproteinsynthesisinhamsters.Methods:Hamstersweretreatedseparatelywiththreediferentregimens:(1)40IUofpregnantmare′sserumgonadotropin(PMSG)folowedby100IUofhCGafter52hours(PMSGalonegroup).(2)Samevolumesofnormalsalineonthesameschedule(controlgroup).(3)40μgofLAdailypriortothesameregimenofPMSG-hCGtreatmentinordertodown-regulatethepituitary-ovarianfunctions(LA-PMSGgroup).Thenewlysynthesizedendometrialproteinswerecharacterizedandquantitatedinvitroby3H-leucineincorporationtestand10%SDS-PAGEtechnique.Results:Thelevelsofnewlysynthesized3H-labeledendometrialproteinswerenotdiferentbetweenLA-PMSGandcontrolgroups.However,thoseoflowandmiddlemolecularweight(<31000and45000)proteinsinthePMSGalonegroupweresignificantlylowerthanthelevelsinbothLA-PMSGandcontrolgroups(P<0.05,P<0.01).Conclusions:TheadditionofLAinovarianstimulationprotocolresultedinmorephysiologicalendometrialproteinsynthesis.Thisphenomenonmaycausalyrelatedtohigherpregnancyratewhenappliedinvitrofertilization-embryotransferprograms.

关 键 词：亮丙瑞林 排卵诱导 子宫内膜 雌二醇 胚胎移植 

分 类 号：R321.33[医药卫生—人体解剖和组织胚胎学]