小麦条锈菌鉴别寄主抗条锈病基因Yr9的微卫星标记  

Microsatellite Marker Linked with Stripe Rust Resistant Gene Yr9 in Wheat

作　　者：翁东旭[1] 徐世昌[1] 蔺瑞明[1] 万安民[1] 李景鹏[2] 吴立人[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100094 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《Acta Genetica Sinica》2005年第9期937-941,共5页

基　　金：国家"973"计划项目(编号:G2000016200);国家"863"计划项目(编号:2002AA245041);国家自然科学基金项目(编号:30471131;30270867)~~

摘　　要：以含有Yr9的抗条锈病近等基因系Taichung29*6/Yr9及其轮回亲本Taichung29为材料,用目的基因所在1B染色体上32对微卫星引物对其基因组DNA进行PCR扩增,发现引物Xgwm582在近等基因系与轮回亲本间可扩增出特异性DNA片段。经F2代分离群体177个抗、感单株检测证实,该片段位点与抗条锈病基因Yr9紧密连锁,遗传距离为3·7cM,确定Xgwm582可作为抗条锈病基因Yr9的标记。SSR analysis was performed using a wheat near-isogenic line（NIL） Taichuang29 ＊ 6/ Lovrin13,which carried the resistance gene Yr9 against wheat stripe rust and its recurrent parent Taichung29 as materials. After screening with 32 SSR primers on 1 B chromosome, reproducible polymorphic DNA fragment amplified by Xgwm582 was identified. Genetic linkage was tested in 177 segregating F2 plants. The results indicated that microsatellite marker Xgwm582 was linked with gene Yr9 resistant to wheat stripe rust. A genetic distance of 3. 7 cM was calculated.

关 键 词：小麦 抗条锈病基因 Yr9 微卫星标记 

分 类 号：S512.1[农业科学—作物学]