Ⅰ型马立克氏病病毒Md11株pp24基因的真核表达  

Eukaryotic expression of pp24 gene of Md11 strain of marek's disease virus

作　　者：姜世金[1] 杨汉春[2] 崔治中[1] 

机构地区：[1]山东农业大学动物科学院,山东泰安271018 [2]中国农业大学动物医学院,北京100094

出　　处：《中国预防兽医学报》2005年第5期326-328,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30070544)

摘　　要：通过PCR方法扩增MDV Md11株的pp24基因,将其克隆到真核表达载体pcDNA3.1/zeo(+)中。阳性克隆鉴定后,在脂质体作用下转染CEF细胞,用间接免疫荧光试验检测,结果证明阳性克隆在CEF细胞中表达了pp24蛋白。pp24 gene of Marek＇s Disease Virus（MDV） Mdll strain was amplified by polymerase chain reaction and cloned into pcDNA3.1/zeo（ ＋ ）.The recombinant plasmid was transfected into chick embryo fibroblasts（CEF）. Indirect immunofluorescence assay （IFA）showed that pp24 gene expressed the 24 kD phosphorylated protein which can response to Mouse-Anti-GST-pp24 sera.

关 键 词：MDV pp24 转染 IFA 

分 类 号：S852.659.4[农业科学—基础兽医学]