不同CpG-DNA序列对鸡ND HI影响的研究  

Selection of CpG-DNA with the most adjuvant effcet to chicken

作　　者：周顺[1] 张祯涛[1] 王金宝[1] 李俊[1] 宋玉才[1] 刘长龙[1] 徐文[1] 倪娇[1] 

机构地区：[1]莱阳农学院动物科技院,山东莱阳265200

出　　处：《中国预防兽医学报》2005年第5期394-396,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：将16种人工合成的CpG_DNA与新城疫Ⅳ弱毒苗(La Sota株)合用,免疫14日龄雏鸡,于免疫前及免疫后7、14、21天测鸡HI抗体效价,筛选出4种CpG_DNA短序并进行SPF鸡重复试验,结果表明:筛选的4种CpG短序(1.AACGTT AACGTT;2.AACGTC AACGTC;3.AGCGTC AGCGTC;4.AGCGCT AGCGCT)可显著提高鸡体的体液免疫水平。Using the NDV model, 16 CpG-DNA were injected into 14-day chicken with vaccine of NDV Ⅳ. HI assay was performed for detection the specific antibody to NDV in sere from inoculated chicken at 7 th, 14 th and 21 stday after immunization. 4 CpG-DNA were selected and redone to SPF chicken. In conclusion, these 4 CpG motifs（ 1. AACGTTAACGTT; 2. AACGTC AACGTC; 3. AGCGTC AGCGTC;4. ATCGCT AGCGCT ） could efficiendy enhance the humoral immune response.

关 键 词：CPG-DNA 免疫佐剂 鸡 筛选 

分 类 号：S852.659.5[农业科学—基础兽医学]